Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

Makoto Ito, Yohei Ishibashi, Takashi Watanabe, Jun Iwaki, Toyohisa Kurita, Nozomu Okino

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Microbial glycosphingolipid (GSL)-degrading enzymes with unique specificity are useful tools for GSL research. On the other hand, some microbial glycolipids, not only GSLs but also steryl glucosides, are closely related to pathogenicity, and, thus, the metabolism of microbial glycolipids is attracting attention as a target for antibiotics. This chapter describes the assays and utilization of microbial enzymes useful for glycolipid research and those involved in pathogenicity or host immune reactions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages229-256
Number of pages28
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2613
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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