Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube-inorganic composite membrane

T. Itoh, T. Shimomura, Y. Hasegawa, J. Mizuguchi, T. Hanaoka, Akari Hayashi, A. Yamaguchi, N. Teramae, M. Ono, F. Mizukami

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We prepared silica nanotube with nanochannel-wall in the columnar pores of a commercial anodic alumina membrane (F127-MST), and then made up an artificial biomembrane consisting of a hierarchical structure by introducing an enzyme, formaldehyde dehydrogenase, into the silica nanochannels. The formaldehyde dehydrogenase confined in membrane showed a very high activity for the reduction of NAD+ similar to that of the native, besides much higher electron transfer to the electrode than the native. At the same time, we clarified that the encapsulation of enzymes into the nanochannels enables enzymes to accumulate in high density without aggregation and be arranged regularly. These results indicate that F127-MST can provide a favorable method for enzyme immobilization on the electrode.

Original languageEnglish
Pages (from-to)251-256
Number of pages6
JournalJournal of Materials Chemistry
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 7 2011
Externally publishedYes

Fingerprint

glutathione-independent formaldehyde dehydrogenase
UCON 50-HB-5100
Composite membranes
Encapsulation
Formaldehyde
Silicon Dioxide
Nanotubes
Enzymes
Silica
Enzyme immobilization
Membranes
Electrodes
Aluminum Oxide
NAD
Alumina
Agglomeration
Electrons
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Chemistry

Cite this

Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube-inorganic composite membrane. / Itoh, T.; Shimomura, T.; Hasegawa, Y.; Mizuguchi, J.; Hanaoka, T.; Hayashi, Akari; Yamaguchi, A.; Teramae, N.; Ono, M.; Mizukami, F.

In: Journal of Materials Chemistry, Vol. 21, No. 1, 07.01.2011, p. 251-256.

Research output: Contribution to journalArticle

Itoh, T. ; Shimomura, T. ; Hasegawa, Y. ; Mizuguchi, J. ; Hanaoka, T. ; Hayashi, Akari ; Yamaguchi, A. ; Teramae, N. ; Ono, M. ; Mizukami, F. / Assembly of an artificial biomembrane by encapsulation of an enzyme, formaldehyde dehydrogenase, into the nanoporous-walled silica nanotube-inorganic composite membrane. In: Journal of Materials Chemistry. 2011 ; Vol. 21, No. 1. pp. 251-256.
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AU - Mizuguchi, J.

AU - Hanaoka, T.

AU - Hayashi, Akari

AU - Yamaguchi, A.

AU - Teramae, N.

AU - Ono, M.

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