Assembly of MreB filaments on liposome membranes: A synthetic biology approach

Yusuke T. Maeda, Tomoyoshi Nakadai, Jonghyeon Shin, Kunihiro Uryu, Vincent Noireaux, Albert Libchaber

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The physical interaction between the cytoskeleton and the cell membrane is essential in defining the morphology of living organisms. In this study, we use a synthetic approach to polymerize bacterial MreB filaments inside phospholipid vesicles. When the proteins MreB and MreC are expressed inside the liposomes, the MreB cytoskeleton structure develops at the inner membrane. Furthermore, when purified MreB is used inside the liposomes, MreB filaments form a 4-10 μm rigid bundle structure and deform the lipid vesicles in physical contact with the vesicle inner membrane. These results indicate that the fibrillation of MreB filaments can take place either in close proximity of deformable lipid membrane or in the presence of associated protein. Our finding might be relevant for the self-assembly of cytoskeleton filaments toward the construction of artificial cell systems.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalACS Synthetic Biology
Volume1
Issue number2
DOIs
Publication statusPublished - Feb 17 2012
Externally publishedYes

Fingerprint

Synthetic Biology
Liposomes
Cytoskeleton
Proteins
Membranes
Rigid structures
Phospholipids
Cell membranes
Membrane Lipids
Self assembly
Lipids
Artificial Cells
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Assembly of MreB filaments on liposome membranes : A synthetic biology approach. / Maeda, Yusuke T.; Nakadai, Tomoyoshi; Shin, Jonghyeon; Uryu, Kunihiro; Noireaux, Vincent; Libchaber, Albert.

In: ACS Synthetic Biology, Vol. 1, No. 2, 17.02.2012, p. 53-59.

Research output: Contribution to journalArticle

Maeda, YT, Nakadai, T, Shin, J, Uryu, K, Noireaux, V & Libchaber, A 2012, 'Assembly of MreB filaments on liposome membranes: A synthetic biology approach', ACS Synthetic Biology, vol. 1, no. 2, pp. 53-59. https://doi.org/10.1021/sb200003v
Maeda, Yusuke T. ; Nakadai, Tomoyoshi ; Shin, Jonghyeon ; Uryu, Kunihiro ; Noireaux, Vincent ; Libchaber, Albert. / Assembly of MreB filaments on liposome membranes : A synthetic biology approach. In: ACS Synthetic Biology. 2012 ; Vol. 1, No. 2. pp. 53-59.
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