TY - JOUR
T1 - Assessment of allelopathic activities in female and male individuals of asparagus seedlings and regenerants
AU - Watanabe, Yasuki
AU - Kuchi-ishi, Natsuki
AU - Nakashima, Toshiki
AU - Iwai, Sumio
AU - Ono, Yoshitaka
AU - Hiradate, Syuntaro
AU - Fujii, Yoshiharu
AU - Komai, Fuminori
PY - 2011
Y1 - 2011
N2 - An aseptic bioassay utilizing female and male donor materials was performed in order to assess the allelopathic activities of cladophylls and root systems in dioecious asparagus. The aseptic sandwich method revealed that the growth of acceptor plants was significantly inhibited by the addition of over 30mg fresh weight of cladophylls. When female and male cladophylls were separately adopted for the sandwich method, there was no sexual difference in the allelopathic activity of both youthful and senescent cladophylls. To detect the allelopathic effects of root systems, the plant box method employing female and male regenerants was used aseptically, and the growth of acceptor plants was seriously inhibited irrespective of sex expression in donor plants. Early in vitro flowering seedlings expressing individual sex were effectively used for the bioassay, and the results indicated that the degree of allelopathic activities was the same as that in regenerants. These results show that allelopathic activity in female and male plants was equivalent, and that the application of materials that can distinguish between the sexes would be useful for assaying the mutual effects of sexuality on allelopathy. The aseptic bioassay system that employed female and male materials presented here could promote the isolation and identification of allelochemicals. JSHS
AB - An aseptic bioassay utilizing female and male donor materials was performed in order to assess the allelopathic activities of cladophylls and root systems in dioecious asparagus. The aseptic sandwich method revealed that the growth of acceptor plants was significantly inhibited by the addition of over 30mg fresh weight of cladophylls. When female and male cladophylls were separately adopted for the sandwich method, there was no sexual difference in the allelopathic activity of both youthful and senescent cladophylls. To detect the allelopathic effects of root systems, the plant box method employing female and male regenerants was used aseptically, and the growth of acceptor plants was seriously inhibited irrespective of sex expression in donor plants. Early in vitro flowering seedlings expressing individual sex were effectively used for the bioassay, and the results indicated that the degree of allelopathic activities was the same as that in regenerants. These results show that allelopathic activity in female and male plants was equivalent, and that the application of materials that can distinguish between the sexes would be useful for assaying the mutual effects of sexuality on allelopathy. The aseptic bioassay system that employed female and male materials presented here could promote the isolation and identification of allelochemicals. JSHS
UR - http://www.scopus.com/inward/record.url?scp=79955490808&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955490808&partnerID=8YFLogxK
U2 - 10.2503/jjshs1.80.169
DO - 10.2503/jjshs1.80.169
M3 - Article
AN - SCOPUS:79955490808
VL - 80
SP - 169
EP - 174
JO - Horticulture Journal
JF - Horticulture Journal
SN - 2189-0102
IS - 2
ER -