Assessment of Allelopathic Activities in Female and Male Individuals of Asparagus Seedlings and Regenerants

Yasuki Watanabe, Natsuki Kuchi-ishi, Toshiki Nakashima, Sumio Iwai, Yoshitaka Ono, Syuntaro Hiradate, Yoshiharu Fujii, Fuminori Komai

Research output: Contribution to journalArticle

Abstract

An aseptic bioassay utilizing female and male donor materials was performed in order to assess the allelopathic activities of cladophylls and root systems in dioecious asparagus. The aseptic sandwich method revealed that the growth of acceptor plants was significantly inhibited by the addition of over 30 mg fresh weight of cladophylls. When female and male cladophylls were separately adopted for the sandwich method, there was no sexual difference in the allelopathic activity of both youthful and senescent cladophylls. To detect the allelopathic effects of root systems, the plant box method employing female and male regenerants was used aseptically, and the growth of acceptor plants was seriously inhibited irrespective of sex expression in donor plants. Early <i>in vitro</i> flowering seedlings expressing individual sex were effectively used for the bioassay, and the results indicated that the degree of allelopathic activities was the same as that in regenerants. These results show that allelopathic activity in female and male plants was equivalent, and that the application of materials that can distinguish between the sexes would be useful for assaying the mutual effects of sexuality on allelopathy. The aseptic bioassay system that employed female and male materials presented here could promote the isolation and identification of allelochemicals.<br>
Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalJournal of the Japanese Society for Horticultural Science
Volume80
Issue number2
DOIs
Publication statusPublished - 2011

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allelopathy
cladodes
seedlings
bioassays
sandwiches
root systems
gender
allelochemicals
methodology
flowering

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Assessment of Allelopathic Activities in Female and Male Individuals of Asparagus Seedlings and Regenerants. / Watanabe, Yasuki; Kuchi-ishi, Natsuki; Nakashima, Toshiki; Iwai, Sumio; Ono, Yoshitaka; Hiradate, Syuntaro; Fujii, Yoshiharu; Komai, Fuminori.

In: Journal of the Japanese Society for Horticultural Science, Vol. 80, No. 2, 2011, p. 169-174.

Research output: Contribution to journalArticle

Watanabe, Yasuki ; Kuchi-ishi, Natsuki ; Nakashima, Toshiki ; Iwai, Sumio ; Ono, Yoshitaka ; Hiradate, Syuntaro ; Fujii, Yoshiharu ; Komai, Fuminori. / Assessment of Allelopathic Activities in Female and Male Individuals of Asparagus Seedlings and Regenerants. In: Journal of the Japanese Society for Horticultural Science. 2011 ; Vol. 80, No. 2. pp. 169-174.
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