Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics

Ryusei Tanaka, Akina Hino, Isheng J. Tsai, Juan Emilio Palomares-Rius, Ayako Yoshida, Yoshitoshi Ogura, Tetsuya Hayashi, Haruhiko Maruyama, Taisei Kikuchi

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26 Citations (Scopus)

Abstract

Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis has been restricted because of the difficult, highly specialised and time-consuming processes involved in parasite identification. In this study, we assessed parasite diversity in wild rats using 18S rDNA-based metagenomics. 18S rDNA PCR products were sequenced using an Illumina MiSeq sequencer and the analysis of the sequences using the QIIME software successfully classified them into several parasite groups. The comparison of the results with those obtained using standard methods including microscopic observation of helminth parasites in the rat intestines and PCR amplification/sequencing of 18S rDNA from isolated single worms suggests that this new technique is reliable and useful to investigate parasite diversity.

Original languageEnglish
Article numbere110769
JournalPloS one
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 23 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Tanaka, R., Hino, A., Tsai, I. J., Palomares-Rius, J. E., Yoshida, A., Ogura, Y., Hayashi, T., Maruyama, H., & Kikuchi, T. (2014). Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics. PloS one, 9(10), [e110769]. https://doi.org/10.1371/journal.pone.0110769