Assessment of rapid diagnostic kit for detecting human adenovirus causing epidemic keratoconjunctivitis

Mei Hashizume, Koki Aoki, Nobuyo Yawata, Gabriel Gonzalez, Susumu Ishida, Shigeaki Ohno, Seiichi Sato, Akinori Takaoka, Nobuyoshi Kitaichi

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Ocular adenovirus infections commonly present clinically with epidemic keratoconjunctivitis (EKC) and pharyngoconjunctival fever (PCF). Their clinical diagnosis is routinely made with immunochromatography (IC) kits, variable accuracy. In this study, the sensitivity of 4 commercial kits against 7 types of human adenoviuses (HAdV) was evaluated in vitro. Methods : HAdV responsible for EKC (types of 8, 37, 53, 54, 56, and 64) and PCF (type 3) were enrolled. Viral solutions were prepared by the serial dilution method and were injected into 4 different commercial kits: 3 Japanese brands (A, B and C) and an American kit (D). The minimum detectable quantity was evaluated in consideration of viral DNA copy numbers and their protein levels. Results: The highest detection sensitivity was shown by kit C followed by kits B, D, and A. New types of HAdV-53, 54, and 56 were detectable in low concentration compared to other types by using the IC kit. Significantly higher viral protein levels were found in the solutions of these 3 types of HAdV than in those of others. HAdV 8 was detectable with low concentration of viral protein. Conclusions: Detection sensitivity limits were different for HAdV and kits. Sensitivities of adenoviral rapid diagnosis tests may be antibodies maybe determined by viral protein level per DNA unit and affinity for HAdV.

Original languageEnglish
Pages (from-to)442-447
Number of pages6
JournalJapanese Journal of Clinical Ophthalmology
Volume75
Issue number4
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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