Association between melanopsin gene polymorphism (I394T) and pupillary light reflex is dependent on light wavelength

Sang il Lee, Akiko Hida, Sei ichi Tsujimura, Takeshi Morita, Kazuo Mishima, Shigekazu Higuchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Our aim was to determine the association between melanopsin gene polymorphism and pupillary light reflex under diverse photic conditions, including different intensities and wavelengths.Methods: A total of 195 visually corrected subjects volunteered for investigation of the melanopsin gene of single nucleotide polymorphism (SNP) of rs1079610 (I394T). The genotype groups were TT (n = 126), TC (n = 55), and CC (n = 8), and 75 of the subjects, including subjects with TT (n = 34), TC (n = 33), and CC (n = 8) participated in our experiment. Three monochromatic lights with peak wavelengths of 465 nm (blue), 536 nm (green), and 632 nm (red) were prepared, and each light was projected to the subjects with five intensities, 12, 13, 14, 14.5 and 15 log photons/(cm2 s), for one minute. The pupil size of the left eye was measured under each light condition after a 1-minute adaptation.Results: The pupils of the TC + CC genotypes (n = 38) were significantly smaller than those of the TT genotype (n = 31) under a blue (463 nm) light condition with 15 log photons/(cm2 s) (P < 0.05). In contrast, there were no significant differences under green (536 nm) and red (632 nm) light conditions. Conversely, relative pupil constrictions of the TC + CC genotypes were greater than those of the TT genotype under both blue and green conditions with high intensities (14.5 and 15 log photons/(cm2 s)). In contrast, there were no significant differences between genotype groups in pupil size and relative pupilloconstriction under the red light conditions.Conclusions: Our findings suggest that the melanopsin gene polymorphism (I394T) functionally interacts with pupillary light reflex, depending on light intensity and, particularly, wavelength, and that under a light condition fulfilling both high intensity and short wavelength, the pupillary light response of subjects with the C allele (TC + CC) is more sensitive to light than that of subjects with the TT genotype.

Original languageEnglish
Article number16
JournalJournal of physiological anthropology
Volume32
Issue number1
DOIs
Publication statusPublished - Oct 12 2013

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Pupillary Reflex
Polymorphism
Genes
Light
Wavelength
pupil
Genotype
Pupil
Photons
melanopsin
Group
experiment
Nucleotides
Constriction
Single Nucleotide Polymorphism

All Science Journal Classification (ASJC) codes

  • Human Factors and Ergonomics
  • Physiology
  • Orthopedics and Sports Medicine
  • Anthropology
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)

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Association between melanopsin gene polymorphism (I394T) and pupillary light reflex is dependent on light wavelength. / Lee, Sang il; Hida, Akiko; Tsujimura, Sei ichi; Morita, Takeshi; Mishima, Kazuo; Higuchi, Shigekazu.

In: Journal of physiological anthropology, Vol. 32, No. 1, 16, 12.10.2013.

Research output: Contribution to journalArticle

Lee, Sang il ; Hida, Akiko ; Tsujimura, Sei ichi ; Morita, Takeshi ; Mishima, Kazuo ; Higuchi, Shigekazu. / Association between melanopsin gene polymorphism (I394T) and pupillary light reflex is dependent on light wavelength. In: Journal of physiological anthropology. 2013 ; Vol. 32, No. 1.
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abstract = "Background: Our aim was to determine the association between melanopsin gene polymorphism and pupillary light reflex under diverse photic conditions, including different intensities and wavelengths.Methods: A total of 195 visually corrected subjects volunteered for investigation of the melanopsin gene of single nucleotide polymorphism (SNP) of rs1079610 (I394T). The genotype groups were TT (n = 126), TC (n = 55), and CC (n = 8), and 75 of the subjects, including subjects with TT (n = 34), TC (n = 33), and CC (n = 8) participated in our experiment. Three monochromatic lights with peak wavelengths of 465 nm (blue), 536 nm (green), and 632 nm (red) were prepared, and each light was projected to the subjects with five intensities, 12, 13, 14, 14.5 and 15 log photons/(cm2 s), for one minute. The pupil size of the left eye was measured under each light condition after a 1-minute adaptation.Results: The pupils of the TC + CC genotypes (n = 38) were significantly smaller than those of the TT genotype (n = 31) under a blue (463 nm) light condition with 15 log photons/(cm2 s) (P < 0.05). In contrast, there were no significant differences under green (536 nm) and red (632 nm) light conditions. Conversely, relative pupil constrictions of the TC + CC genotypes were greater than those of the TT genotype under both blue and green conditions with high intensities (14.5 and 15 log photons/(cm2 s)). In contrast, there were no significant differences between genotype groups in pupil size and relative pupilloconstriction under the red light conditions.Conclusions: Our findings suggest that the melanopsin gene polymorphism (I394T) functionally interacts with pupillary light reflex, depending on light intensity and, particularly, wavelength, and that under a light condition fulfilling both high intensity and short wavelength, the pupillary light response of subjects with the C allele (TC + CC) is more sensitive to light than that of subjects with the TT genotype.",
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T1 - Association between melanopsin gene polymorphism (I394T) and pupillary light reflex is dependent on light wavelength

AU - Lee, Sang il

AU - Hida, Akiko

AU - Tsujimura, Sei ichi

AU - Morita, Takeshi

AU - Mishima, Kazuo

AU - Higuchi, Shigekazu

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AB - Background: Our aim was to determine the association between melanopsin gene polymorphism and pupillary light reflex under diverse photic conditions, including different intensities and wavelengths.Methods: A total of 195 visually corrected subjects volunteered for investigation of the melanopsin gene of single nucleotide polymorphism (SNP) of rs1079610 (I394T). The genotype groups were TT (n = 126), TC (n = 55), and CC (n = 8), and 75 of the subjects, including subjects with TT (n = 34), TC (n = 33), and CC (n = 8) participated in our experiment. Three monochromatic lights with peak wavelengths of 465 nm (blue), 536 nm (green), and 632 nm (red) were prepared, and each light was projected to the subjects with five intensities, 12, 13, 14, 14.5 and 15 log photons/(cm2 s), for one minute. The pupil size of the left eye was measured under each light condition after a 1-minute adaptation.Results: The pupils of the TC + CC genotypes (n = 38) were significantly smaller than those of the TT genotype (n = 31) under a blue (463 nm) light condition with 15 log photons/(cm2 s) (P < 0.05). In contrast, there were no significant differences under green (536 nm) and red (632 nm) light conditions. Conversely, relative pupil constrictions of the TC + CC genotypes were greater than those of the TT genotype under both blue and green conditions with high intensities (14.5 and 15 log photons/(cm2 s)). In contrast, there were no significant differences between genotype groups in pupil size and relative pupilloconstriction under the red light conditions.Conclusions: Our findings suggest that the melanopsin gene polymorphism (I394T) functionally interacts with pupillary light reflex, depending on light intensity and, particularly, wavelength, and that under a light condition fulfilling both high intensity and short wavelength, the pupillary light response of subjects with the C allele (TC + CC) is more sensitive to light than that of subjects with the TT genotype.

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