Abstract
Background: The effect of preformed antidonor antibodies have been demonstrated in various types of solid organ transplantation. However, the significance of anti-donor antibodies in intestinal transplantation remains unclear. The aim of this study is to evaluate the impact that the extent of T cell crossmatch has on the outcome of swine intestinal transplantation. Materials and methods: All studies were performed on outbred domestic male pigs weighing from 15 to 20 kg. Intestinal transplantation was performed orthotopically with an exchange of grafts between white and black pigs. FK506 was administered intravenously (0.1 mg/kg per day, POD 0-7) for immunosuppression. A lymphocyte crossmatch test was performed using the direct CDC crossmatch. The results were considered positive when more than 10% of the donor lymphocytes were killed by the recipient's serum. In addition, 0-10, 11-20, 21-30, 31-80 and 81-100% of the killed lymphocytes were classified as grade 1, 2, 4, 6 and 8, respectively. Result: A total of 34 intestinal transplantations were performed. All but one case had positive donor specific T cell crossmatches. The number of grade 2, 4, 6 and 8 cases was 11, 14, 6 and 2, respectively. Although there was a tendency towards a decreased survival according to the grade, the survival rate was not statistically different among each different grade. Moreover, the rates of acute cellular rejection and vascular complications were not significantly different among the four grades. Conclusion: These results suggest that the extent of positive T cell crossmatch is not associated with the outcome of swine intestinal transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 279-281 |
| Number of pages | 3 |
| Journal | Pediatric surgery international |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1 2011 |
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All Science Journal Classification (ASJC) codes
- Pediatrics, Perinatology, and Child Health
- Surgery
Cite this
Association of lymphocyte crossmatch and the outcome of intestinal transplantation in swine. / Hayashida, Makoto; Matsuura, Toshiharu; Saeki, Isamu; Yanagi, Yusuke; Yoshimaru, Koichiro; Nishimoto, Yuko; Takahashi, Yukiko; Fujita, Keiko; Takada, Narito; Taguchi, Shohei; Uesugi, Toru; Hirose, Ryuichiro; Nakamura, Masatoshi; Nakao, Makoto; Taguchi, Tomoaki.
In: Pediatric surgery international, Vol. 27, No. 3, 01.03.2011, p. 279-281.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of lymphocyte crossmatch and the outcome of intestinal transplantation in swine
AU - Hayashida, Makoto
AU - Matsuura, Toshiharu
AU - Saeki, Isamu
AU - Yanagi, Yusuke
AU - Yoshimaru, Koichiro
AU - Nishimoto, Yuko
AU - Takahashi, Yukiko
AU - Fujita, Keiko
AU - Takada, Narito
AU - Taguchi, Shohei
AU - Uesugi, Toru
AU - Hirose, Ryuichiro
AU - Nakamura, Masatoshi
AU - Nakao, Makoto
AU - Taguchi, Tomoaki
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Background: The effect of preformed antidonor antibodies have been demonstrated in various types of solid organ transplantation. However, the significance of anti-donor antibodies in intestinal transplantation remains unclear. The aim of this study is to evaluate the impact that the extent of T cell crossmatch has on the outcome of swine intestinal transplantation. Materials and methods: All studies were performed on outbred domestic male pigs weighing from 15 to 20 kg. Intestinal transplantation was performed orthotopically with an exchange of grafts between white and black pigs. FK506 was administered intravenously (0.1 mg/kg per day, POD 0-7) for immunosuppression. A lymphocyte crossmatch test was performed using the direct CDC crossmatch. The results were considered positive when more than 10% of the donor lymphocytes were killed by the recipient's serum. In addition, 0-10, 11-20, 21-30, 31-80 and 81-100% of the killed lymphocytes were classified as grade 1, 2, 4, 6 and 8, respectively. Result: A total of 34 intestinal transplantations were performed. All but one case had positive donor specific T cell crossmatches. The number of grade 2, 4, 6 and 8 cases was 11, 14, 6 and 2, respectively. Although there was a tendency towards a decreased survival according to the grade, the survival rate was not statistically different among each different grade. Moreover, the rates of acute cellular rejection and vascular complications were not significantly different among the four grades. Conclusion: These results suggest that the extent of positive T cell crossmatch is not associated with the outcome of swine intestinal transplantation.
AB - Background: The effect of preformed antidonor antibodies have been demonstrated in various types of solid organ transplantation. However, the significance of anti-donor antibodies in intestinal transplantation remains unclear. The aim of this study is to evaluate the impact that the extent of T cell crossmatch has on the outcome of swine intestinal transplantation. Materials and methods: All studies were performed on outbred domestic male pigs weighing from 15 to 20 kg. Intestinal transplantation was performed orthotopically with an exchange of grafts between white and black pigs. FK506 was administered intravenously (0.1 mg/kg per day, POD 0-7) for immunosuppression. A lymphocyte crossmatch test was performed using the direct CDC crossmatch. The results were considered positive when more than 10% of the donor lymphocytes were killed by the recipient's serum. In addition, 0-10, 11-20, 21-30, 31-80 and 81-100% of the killed lymphocytes were classified as grade 1, 2, 4, 6 and 8, respectively. Result: A total of 34 intestinal transplantations were performed. All but one case had positive donor specific T cell crossmatches. The number of grade 2, 4, 6 and 8 cases was 11, 14, 6 and 2, respectively. Although there was a tendency towards a decreased survival according to the grade, the survival rate was not statistically different among each different grade. Moreover, the rates of acute cellular rejection and vascular complications were not significantly different among the four grades. Conclusion: These results suggest that the extent of positive T cell crossmatch is not associated with the outcome of swine intestinal transplantation.
UR - http://www.scopus.com/inward/record.url?scp=79954438340&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79954438340&partnerID=8YFLogxK
U2 - 10.1007/s00383-010-2796-x
DO - 10.1007/s00383-010-2796-x
M3 - Article
C2 - 21069345
AN - SCOPUS:79954438340
VL - 27
SP - 279
EP - 281
JO - Pediatric Surgery International
JF - Pediatric Surgery International
SN - 0179-0358
IS - 3
ER -