Association of Ob-R Gene Polymorphism and Insulin Resistance in Japanese Men

Akiko Takahashi-Yasuno, Hiroaki Masuzaki, Takashi Miyawaki, Naoki Matsuoka, Yoshihiro Ogawa, Tatsuya Hayashi, Kiminori Hosoda, Yasunao Yoshimasa, Gen Inoue, Kazuwa Nakao

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Leptin and its receptors are known to play a role in glucose metabolism. We succeeded in cloning human Ob-R cDNA and revealed 7 single nucleotide polymorphisms (SNPs) (Lys109Arg, Arg223Gln, Ser343Ser, Ser492Thr, Lys656Asn, Ala976Asp, and Pro1019Pro) in the coding region of Ob-Rb. Although these 7 SNPs were not associated with an obese phenotype, several studies have reported that some of them were associated with impaired glucose metabolism. To clarify whether the Arg223Gln and A3057G (Pro1019Pro) polymorphisms influence glucose metabolism in Japanese, 696 Japanese men were genotyped. Individually, the Arg223Gln and the A3057G polymorphisms were not associated with the glucose metabolic parameters. No associations were found between haplotype and clinical parameters. However, in 327 subjects with normal glucose tolerance (NGT), the subjects with Arg/Gln or Gln/Gln + A/A haplotype showed significantly higher serum insulin levels and homeostasis model assessment (HOMA) index than those with Arg/Arg + A/A haplotype and Arg/Gln or Gln/Gln + A/G or G/G haplotype. The subjects with Arg/Gln or Gln/Gln + A/A haplotype showed a significantly lower fasting glucose to insulin (GI) ratio than those with Arg/Arg + A/A haplotype. These results suggest that the Ob-R gene may serve as a modifier gene for insulin resistance in Japanese men.

Original languageEnglish
Pages (from-to)650-654
Number of pages5
JournalMetabolism: Clinical and Experimental
Volume53
Issue number5
DOIs
Publication statusPublished - May 2004

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vpr Genes
Haplotypes
Insulin Resistance
Glucose
Single Nucleotide Polymorphism
Modifier Genes
Insulin
Leptin Receptors
Organism Cloning
Fasting
Homeostasis
Complementary DNA
Phenotype
Serum

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Takahashi-Yasuno, A., Masuzaki, H., Miyawaki, T., Matsuoka, N., Ogawa, Y., Hayashi, T., ... Nakao, K. (2004). Association of Ob-R Gene Polymorphism and Insulin Resistance in Japanese Men. Metabolism: Clinical and Experimental, 53(5), 650-654. https://doi.org/10.1016/j.metabol.2003.12.012

Association of Ob-R Gene Polymorphism and Insulin Resistance in Japanese Men. / Takahashi-Yasuno, Akiko; Masuzaki, Hiroaki; Miyawaki, Takashi; Matsuoka, Naoki; Ogawa, Yoshihiro; Hayashi, Tatsuya; Hosoda, Kiminori; Yoshimasa, Yasunao; Inoue, Gen; Nakao, Kazuwa.

In: Metabolism: Clinical and Experimental, Vol. 53, No. 5, 05.2004, p. 650-654.

Research output: Contribution to journalArticle

Takahashi-Yasuno, A, Masuzaki, H, Miyawaki, T, Matsuoka, N, Ogawa, Y, Hayashi, T, Hosoda, K, Yoshimasa, Y, Inoue, G & Nakao, K 2004, 'Association of Ob-R Gene Polymorphism and Insulin Resistance in Japanese Men', Metabolism: Clinical and Experimental, vol. 53, no. 5, pp. 650-654. https://doi.org/10.1016/j.metabol.2003.12.012
Takahashi-Yasuno, Akiko ; Masuzaki, Hiroaki ; Miyawaki, Takashi ; Matsuoka, Naoki ; Ogawa, Yoshihiro ; Hayashi, Tatsuya ; Hosoda, Kiminori ; Yoshimasa, Yasunao ; Inoue, Gen ; Nakao, Kazuwa. / Association of Ob-R Gene Polymorphism and Insulin Resistance in Japanese Men. In: Metabolism: Clinical and Experimental. 2004 ; Vol. 53, No. 5. pp. 650-654.
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