Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae

Yuko Todaka, Yonggang Wang, Kosuke Tashiro, Nobutaka Nakashima, Takeharu Nishimoto, Takeshi Sekiguchi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Yeast Gtr1p and its human homolog RRAG A belong to the Ras-like small G-protein superfamily and genetically interact with RCC1, a guanine nucleotide exchange factor for Ran GTPase. Little is known regarding the function of Gtr1p. We performed yeast two-hybrid screening using Gtr1p as the bait to find interacting proteins. Rpc19p, a shared subunit of RNA polymerases I and III, associated with Gtr1p. The association of Gtr1p with Rpc19p occurred in a GTP-form-specific manner. RRAG A associated with RPA16 (human Rpc19p homolog) in a GTP-form-specific manner, suggesting that the association is conserved during evolution. Ribosomal RNA and tRNA synthesis were reduced in the gtr1Δ strain expressing the GDP form of Gtr1p, but not the GTP form of Gtr1p. Gel-filtration studies revealed an accumulation of the smaller Rpc19p-containing complex, but not of A135, in the gtr1Δ strain. Here, we propose that Gtr1p is involved in RNA polymerase I and III assembly by its association with Rpc19p and could be a mediator that links growth regulatory signals with ribosome biogenesis.

Original languageEnglish
Pages (from-to)1515-1524
Number of pages10
JournalGenetics
Volume170
Issue number4
DOIs
Publication statusPublished - Aug 1 2005

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RNA Polymerase I
RNA Polymerase III
Guanosine Triphosphate
GTP-Binding Proteins
Saccharomyces cerevisiae
Yeasts
Guanine Nucleotide Exchange Factors
Ribosomal RNA
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Transfer RNA
Ribosomes
Gel Chromatography
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae. / Todaka, Yuko; Wang, Yonggang; Tashiro, Kosuke; Nakashima, Nobutaka; Nishimoto, Takeharu; Sekiguchi, Takeshi.

In: Genetics, Vol. 170, No. 4, 01.08.2005, p. 1515-1524.

Research output: Contribution to journalArticle

Todaka, Yuko ; Wang, Yonggang ; Tashiro, Kosuke ; Nakashima, Nobutaka ; Nishimoto, Takeharu ; Sekiguchi, Takeshi. / Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae. In: Genetics. 2005 ; Vol. 170, No. 4. pp. 1515-1524.
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AU - Nakashima, Nobutaka

AU - Nishimoto, Takeharu

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