Association of the IL12RB1 promoter polymorphisms with increased risk of atopic dermatitis and other allergic phenotypes

Naomi Takahashi, Mitsuteru Akahoshi, Akira Matsuda, Kouji Ebe, Naoko Inomata, Kazuhiko Obara, Tomomitsu Hirota, Kazuko Nakashima, Makiko Shimizu, Mayumi Tamari, Satoru Doi, Akihiko Miyatake, Tadao Enomoto, Hitoshi Nakashima, Zenro Ikezawa, Taro Shirakawa

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38 Citations (Scopus)

Abstract

Atopic dermatitis (AD) is frequently associated with eosinophilia, highly elevated immunoglobulin E (IgE) levels and increased levels of T-helper 2-type (Th2) cytokines in skin lesions due to infiltrating T cells. Interleukin-12 (IL-12), in combination with interferon-γ (IFN-γ), inhibits IgE synthesis and Th2 cell function. As the IFN-γ-inducing cytokines IL-12 and IL-23 utilize IL-12Rβ1 as part of their receptors, it is possible that polymorphic variants of the IL-12Rβ1 (IL12RB1) gene might determine an individual's susceptibility to AD. Here, we carried out a systemic search for genetic variants of the human IL12RB1 in Japanese subjects and identified 48 genetic variants. In a case-control association study, we found that promoter polymorphisms - 111A/T and - 2C/T were significantly associated with an increased risk of AD under a recessive model. The - 111T-allele frequency in the independent population of child asthmatics was also much higher than that in the control group. In addition, the - 111T/T genotype was progressively more common in AD with high total serum IgE levels in an IgE-level-dependent manner. Deletion analysis of the IL12RB1 promoter suggested that the -265 to -104 region that contained the -111A/T polymorphic site harbored an important regulatory element. Furthermore, we showed that the -111A/T substitution appeared to cause decreased gene transcriptional activity such that cells from -111A/A individuals exhibited higher IL12RB1 mRNA levels than those from -111T allele carriers. Our results suggested that in individuals with the -111T/T genotype, reduced IL-12Rβ1 expression may lead to increased Th2 cytokine production in the skin and contribute to the development of AD and other subsequent allergic diseases.

Original languageEnglish
Pages (from-to)3149-3159
Number of pages11
JournalHuman Molecular Genetics
Volume14
Issue number21
DOIs
Publication statusPublished - Nov 1 2005

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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