TY - JOUR
T1 - Astrocytic Ca2+ responses in the spinal dorsal horn by noxious stimuli to the skin
AU - Yoshihara, Kohei
AU - Matsuda, Tsuyoshi
AU - Kohro, Yuta
AU - Tozaki-Saitoh, Hidetoshi
AU - Inoue, Kazuhide
AU - Tsuda, Makoto
N1 - Funding Information:
We thank the University of Pennsylvania vector core for providing pZac2.1, pAAV2/9 and pAd DeltaF6 plasmid. This work was supported by Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) grant numbers JP15H02522 (M.T.), JP25117013 (K.I.), the Core Research for Evolutional Science and Technology (CREST) program under Grant Number JP17gm0910006 (M.T.), the Practical Research Project for Allergic Diseases and Immunology (Research on Allergic Diseases and Immunology) under Grant Number JP17ek0410034 (M.T.), the Research Project on Elucidation of Chronic Pain (M.T., H.T.-S.), the Platform Project for Supporting Drug Discovery and Life Science Research (BINDS) under Grant Number JP18am0101091 (M.T.) from AMED, and the Toray Science Foundation (M.T.). T.M. is a research fellow of the JSPS (15J03522).
Funding Information:
We thank the University of Pennsylvania vector core for providing pZac2.1, pAAV2/9 and pAd DeltaF6 plasmid. This work was supported by Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) grant numbers JP15H02522 (M.T.) , JP25117013 (K.I.) , the Core Research for Evolutional Science and Technology (CREST) program under Grant Number JP17gm0910006 (M.T.) , the Practical Research Project for Allergic Diseases and Immunology (Research on Allergic Diseases and Immunology) under Grant Number JP17ek0410034 (M.T.) , the Research Project on Elucidation of Chronic Pain (M.T., H.T.-S.), the Platform Project for Supporting Drug Discovery and Life Science Research (BINDS) under Grant Number JP18am0101091 (M.T.) from AMED, and the Toray Science Foundation (M.T.). T.M. is a research fellow of the JSPS (15J03522).
Publisher Copyright:
© 2018 The Authors
PY - 2018/5
Y1 - 2018/5
N2 - The role of astrocytes in the spinal dorsal horn (SDH) for sensory information processing under normal conditions is poorly understood. In this study, we investigated whether SDH astrocytes respond to noxious and innocuous stimuli to the skin of normal mice using in vivo two-photon Ca2+ imaging under anesthesia. We found that noxious stimulation evoked by intraplantar formalin injection provoked an elevation in intracellular Ca2+ levels in SDH astrocytes. By contrast, neither instantaneous noxious pinching nor innocuous stimuli (cooling or brushing) to the hindpaw elicited astrocytic Ca2+ responses. Thus, SDH astrocytes could respond preferentially to a strong and/or sustained noxious stimulus.
AB - The role of astrocytes in the spinal dorsal horn (SDH) for sensory information processing under normal conditions is poorly understood. In this study, we investigated whether SDH astrocytes respond to noxious and innocuous stimuli to the skin of normal mice using in vivo two-photon Ca2+ imaging under anesthesia. We found that noxious stimulation evoked by intraplantar formalin injection provoked an elevation in intracellular Ca2+ levels in SDH astrocytes. By contrast, neither instantaneous noxious pinching nor innocuous stimuli (cooling or brushing) to the hindpaw elicited astrocytic Ca2+ responses. Thus, SDH astrocytes could respond preferentially to a strong and/or sustained noxious stimulus.
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U2 - 10.1016/j.jphs.2018.04.007
DO - 10.1016/j.jphs.2018.04.007
M3 - Article
C2 - 29773517
AN - SCOPUS:85047181081
VL - 137
SP - 101
EP - 104
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
SN - 1347-8613
IS - 1
ER -