TY - JOUR
T1 - Astrocytic d-amino acid oxidase degrades d-serine in the hindbrain
AU - Gonda, Yusuke
AU - Ishii, Chiharu
AU - Mita, Masashi
AU - Nishizaki, Naoto
AU - Ohtomo, Yoshiyuki
AU - Hamase, Kenji
AU - Shimizu, Toshiaki
AU - Sasabe, Jumpei
N1 - Funding Information:
This research was funded by Japan Agency for Medical Research and Development (AMED‐PRIME) (19gm6010017h0002) (JS), JSPS KAKENHI Grant number 21H02982 (JS), Shiseido. Co. Ltd. and Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research (JS).
Publisher Copyright:
© 2022 Federation of European Biochemical Societies.
PY - 2022
Y1 - 2022
N2 - d-Serine modulates excitatory neurotransmission by binding to N-methyl-d-aspartate glutamate receptors. d-Amino acid oxidase (DAO) degrades d-amino acids, such as d-serine, in the central nervous system, and is associated with neurological and psychiatric disorders. However, cell types that express brain DAO remain controversial, and whether brain DAO influences systemic d-amino acids in addition to brain d-serine remains unclear. Here, we created astrocyte-specific DAO-conditional knockout mice. Knockout in glial fibrillary acidic protein-positive cells eliminated DAO expression in the hindbrain and increased d-serine levels significantly in the cerebellum. Brain DAO did not influence levels of d-amino acids in the forebrain or periphery. These results show that astrocytic DAO regulates d-serine specifically in the hindbrain.
AB - d-Serine modulates excitatory neurotransmission by binding to N-methyl-d-aspartate glutamate receptors. d-Amino acid oxidase (DAO) degrades d-amino acids, such as d-serine, in the central nervous system, and is associated with neurological and psychiatric disorders. However, cell types that express brain DAO remain controversial, and whether brain DAO influences systemic d-amino acids in addition to brain d-serine remains unclear. Here, we created astrocyte-specific DAO-conditional knockout mice. Knockout in glial fibrillary acidic protein-positive cells eliminated DAO expression in the hindbrain and increased d-serine levels significantly in the cerebellum. Brain DAO did not influence levels of d-amino acids in the forebrain or periphery. These results show that astrocytic DAO regulates d-serine specifically in the hindbrain.
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U2 - 10.1002/1873-3468.14417
DO - 10.1002/1873-3468.14417
M3 - Letter
C2 - 35665501
AN - SCOPUS:85131751571
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
ER -