Asymmetric distribution of acetylcholine receptors and M channels on prepyriform neurons

J. M.H. ffrench-Mullen, N. Hori, H. Nakanishi, N. T. Slater, D. O. Carpenter

Research output: Contribution to journalArticle

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Abstract

1. The responses of pyramidal neurons of rat prepyriform cortex to ionophoretic application of acetylcholine (ACh) were studied in a submerged, perfused brain slice. 2. ACh excited some neurons but only if applied to an area near to the cut surface of the slice. This area contained the basal dendrites of the pyramidal cells and some cell bodies. No excitation was seen if ACh was applied at depths of 250 μm or more from the cut surface, an area which contained only apical dendrites, although the apical dendrites were very sensitive to excitatory amino acids such as aspartate (Asp) and glutamate (Glu). 3. On all neurons which did not discharge to ionophoretic application of ACh, ACh potentiated the response to Glu and Asp. No potentiation of amino acid responses was obtained on apical dendrites. The potentiation had a time course similar to that of the discharge of neurons which fired to ACh. This observation suggests that pyramidal neurons have receptors for ACh on basal but not apical dendrites. 4. The ACh response in the basal dendrite-soma region was elicted by pilocarpine and blocked by atropine but not curare. This was true whether the response studied was direct excitation or potentiation of the response to an amino acid. 5. The ACh response was associated with a voltage-dependent increase in membrane resistance which had a slow time course and appeared to be due to a turning off of an M current, as described by Brown and Adams (1980) in sympathetic ganglion cells. The effects of ACh were minimal at the resting potential but increased with depolarization. ACh had no effect on the current-voltage relation of the cell, except at depolarized potentials of less than -60 mV. 6. Ionophoretic application of Ba2+ to the basal dendritic region resulted in potentiation of the amino acid responses and sometimes induced a discharge similar to that of ACh. Since Ba2+ mimics the ACh response, presumably by a direct blockade of the M channel, the effects of Ba2+ on apical dendrites were tested to determine whether these dendrites contain M channels associated with a transmitter receptor other than ACh. However, Ba2+ did not induce potentiation in apical dendrites, suggesting that M channels are also restricted to the basal dendrites or cell bodies. 7. The potentiation of a response to a depolarizing constant-current pulse was comparable in degree and time course to that of amino acid responses, indicating that the ACh potentiation of the amino acid responses is a predictable augmentation of any input which brings the membrane potential into the range of M-channel activation. 8. The segregation of ACh receptors to a discrete portion of the cell suggests that they are functional receptors and may be part of a physiologic arrangement which directs different afferent inputs to different portions of the dendritic tree. Such segregation of receptors may also occur on other neurons with distributed inputs.

Original languageEnglish
Pages (from-to)163-181
Number of pages19
JournalCellular and Molecular Neurobiology
Volume3
Issue number2
DOIs
Publication statusPublished - Jun 1 1983

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Cholinergic Receptors
Acetylcholine
Dendrites
Neurons
Pyramidal Cells
Amino Acids
Aspartic Acid
Membrane Potentials
Glutamic Acid
Curare
Sympathetic Ganglia
Excitatory Amino Acids
Pilocarpine
Carisoprodol
Atropine

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

ffrench-Mullen, J. M. H., Hori, N., Nakanishi, H., Slater, N. T., & Carpenter, D. O. (1983). Asymmetric distribution of acetylcholine receptors and M channels on prepyriform neurons. Cellular and Molecular Neurobiology, 3(2), 163-181. https://doi.org/10.1007/BF00735280

Asymmetric distribution of acetylcholine receptors and M channels on prepyriform neurons. / ffrench-Mullen, J. M.H.; Hori, N.; Nakanishi, H.; Slater, N. T.; Carpenter, D. O.

In: Cellular and Molecular Neurobiology, Vol. 3, No. 2, 01.06.1983, p. 163-181.

Research output: Contribution to journalArticle

ffrench-Mullen, JMH, Hori, N, Nakanishi, H, Slater, NT & Carpenter, DO 1983, 'Asymmetric distribution of acetylcholine receptors and M channels on prepyriform neurons', Cellular and Molecular Neurobiology, vol. 3, no. 2, pp. 163-181. https://doi.org/10.1007/BF00735280
ffrench-Mullen, J. M.H. ; Hori, N. ; Nakanishi, H. ; Slater, N. T. ; Carpenter, D. O. / Asymmetric distribution of acetylcholine receptors and M channels on prepyriform neurons. In: Cellular and Molecular Neurobiology. 1983 ; Vol. 3, No. 2. pp. 163-181.
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