ATP-activated single-channel currents recorded from cell-free patches of pheochromocytoma PC12 cells

Ken Nakazawa, Kazuhide Inoue, Kannosuke Fujimori, Akira Takanaka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Single-channel recordings were made using cell-free membrane patches (outside-out configuration) isolated from pheochromocytoma PC12 cells. ATP (50 μM) activated single channel currents in the isolated patches and the currents inactivated with a half-decay time of about 5 s. The single channel conductance was about 13 pS in external solution with 140 mM Na. The amplitudes of the single-channel currents were decreased when external Ca was increased from 1.8 to 16.2 mM, suggesting that Ca blocks ion permeation through the channel. These properties of single-channel currents may underlie those of the macroscopic current.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Volume119
Issue number1
DOIs
Publication statusPublished - Oct 30 1990
Externally publishedYes

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PC12 Cells
Pheochromocytoma
Adenosine Triphosphate
Cell Membrane
Ions

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

ATP-activated single-channel currents recorded from cell-free patches of pheochromocytoma PC12 cells. / Nakazawa, Ken; Inoue, Kazuhide; Fujimori, Kannosuke; Takanaka, Akira.

In: Neuroscience Letters, Vol. 119, No. 1, 30.10.1990, p. 5-8.

Research output: Contribution to journalArticle

Nakazawa, Ken ; Inoue, Kazuhide ; Fujimori, Kannosuke ; Takanaka, Akira. / ATP-activated single-channel currents recorded from cell-free patches of pheochromocytoma PC12 cells. In: Neuroscience Letters. 1990 ; Vol. 119, No. 1. pp. 5-8.
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