ATP-activated single-channel currents recorded from cell-free patches of pheochromocytoma PC12 cells

Ken Nakazawa, Kazuhide Inoue, Kannosuke Fujimori, Akira Takanaka

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Single-channel recordings were made using cell-free membrane patches (outside-out configuration) isolated from pheochromocytoma PC12 cells. ATP (50 μM) activated single channel currents in the isolated patches and the currents inactivated with a half-decay time of about 5 s. The single channel conductance was about 13 pS in external solution with 140 mM Na. The amplitudes of the single-channel currents were decreased when external Ca was increased from 1.8 to 16.2 mM, suggesting that Ca blocks ion permeation through the channel. These properties of single-channel currents may underlie those of the macroscopic current.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Volume119
Issue number1
DOIs
Publication statusPublished - Oct 30 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'ATP-activated single-channel currents recorded from cell-free patches of pheochromocytoma PC12 cells'. Together they form a unique fingerprint.

Cite this