ATP-dependent Ca2+ accumulation in intracellular membranes of guinea pig macrophages after saponin treatment

Masato Hirata, Toshitaka Koga

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Ca2+ transport system in the intracellular membranes was studied by using saponin-treated macrophages of the guinea pig, in which the plasma membranes could be selectively destroyed. Saponin-treated macrophages could accumulate 3.1 nmoles Ca2+ 4 × 106 macrophages in the presence of Mg-ATP and sodium azide with an apparent affinity constant of 6 × 106 M-1. In the absence of sodium azide, the value of Ca2+ uptake of saponin-treated macrophages was 95 nmoles/4 × 106 macrophages, and its affinity constant for Ca2+ was 3 × 105 M-1. Saponin-treated macrophages may be suitable for the studies of Ca2+ transport systems in the intracellular membranes.

Original languageEnglish
Pages (from-to)1544-1549
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume104
Issue number4
DOIs
Publication statusPublished - Feb 26 1982

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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