ATP11C is a major flippase in human erythrocytes and its defect causes congenital hemolytic anemia

Nobuto Arashiki, Yuichi Takakuwa, Narla Mohandas, John Hale, Kenichi Yoshida, Hiromi Ogura, Taiju Utsugisawa, Shouichi Ohga, Satoru Miyano, Seishi Ogawa, Seiji Kojima, Hitoshi Kanno

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Phosphatidylserine is localized exclusively to the inner leaflet of the membrane lipid bilayer of most cells, including erythrocytes. This asymmetric distribution is critical for the survival of erythrocytes in circulation since externalized phosphatidylserine is a phagocytic signal for splenic macrophages. Flippases are P-IV ATPase family proteins that actively transport phosphatidylserine from the outer to inner leaflet. It has not yet been determined which of the 14 members of this family of proteins is the flippase in human erythrocytes. Herein, we report that ATP11C encodes a major flippase in human erythrocytes, and a genetic mutation identified in a male patient caused congenital hemolytic anemia inherited as an X-linked recessive trait. Phosphatidylserine internalization in erythrocytes with the mutant ATP11C was decreased 10-fold compared to that of the control, functionally establishing that ATP11C is a major flippase in human erythrocytes. Contrary to our expectations phosphatidylserine was retained in the inner leaflet of the majority of mature erythrocytes from both controls and the patient, suggesting that phosphatidylserine cannot be externalized as long as scramblase is inactive. Phosphatidylserine-exposing cells were found only in the densest senescent cells (0.1% of total) in which scramblase was activated by increased Ca2+ concentration: the percentage of these phosphatidylserine-exposing cells was increased in the patient’s senescent cells accounting for his mild anemia. Furthermore, the finding of similar extents of phosphatidylserine exposure by exogenous Ca2+-activated scrambling in both control erythrocytes and the patient’s erythrocytes implies that suppressed scramblase activity rather than flippase activity contributes to the maintenance of phosphatidylserine in the inner leaflet of human erythrocytes.

Original languageEnglish
Pages (from-to)559-565
Number of pages7
JournalHaematologica
Volume101
Issue number5
DOIs
Publication statusPublished - Apr 30 2016

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Congenital Hemolytic Anemia
Phosphatidylserines
Erythrocytes
Erythrocyte Aging
X-Linked Genes
Lipid Bilayers
Protein Transport
Membrane Lipids
Adenosine Triphosphatases
Anemia

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Arashiki, N., Takakuwa, Y., Mohandas, N., Hale, J., Yoshida, K., Ogura, H., ... Kanno, H. (2016). ATP11C is a major flippase in human erythrocytes and its defect causes congenital hemolytic anemia. Haematologica, 101(5), 559-565. https://doi.org/10.3324/haematol.2016.142273

ATP11C is a major flippase in human erythrocytes and its defect causes congenital hemolytic anemia. / Arashiki, Nobuto; Takakuwa, Yuichi; Mohandas, Narla; Hale, John; Yoshida, Kenichi; Ogura, Hiromi; Utsugisawa, Taiju; Ohga, Shouichi; Miyano, Satoru; Ogawa, Seishi; Kojima, Seiji; Kanno, Hitoshi.

In: Haematologica, Vol. 101, No. 5, 30.04.2016, p. 559-565.

Research output: Contribution to journalArticle

Arashiki, N, Takakuwa, Y, Mohandas, N, Hale, J, Yoshida, K, Ogura, H, Utsugisawa, T, Ohga, S, Miyano, S, Ogawa, S, Kojima, S & Kanno, H 2016, 'ATP11C is a major flippase in human erythrocytes and its defect causes congenital hemolytic anemia', Haematologica, vol. 101, no. 5, pp. 559-565. https://doi.org/10.3324/haematol.2016.142273
Arashiki, Nobuto ; Takakuwa, Yuichi ; Mohandas, Narla ; Hale, John ; Yoshida, Kenichi ; Ogura, Hiromi ; Utsugisawa, Taiju ; Ohga, Shouichi ; Miyano, Satoru ; Ogawa, Seishi ; Kojima, Seiji ; Kanno, Hitoshi. / ATP11C is a major flippase in human erythrocytes and its defect causes congenital hemolytic anemia. In: Haematologica. 2016 ; Vol. 101, No. 5. pp. 559-565.
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AU - Takakuwa, Yuichi

AU - Mohandas, Narla

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AU - Yoshida, Kenichi

AU - Ogura, Hiromi

AU - Utsugisawa, Taiju

AU - Ohga, Shouichi

AU - Miyano, Satoru

AU - Ogawa, Seishi

AU - Kojima, Seiji

AU - Kanno, Hitoshi

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