Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice

Jinmin Zhao, Keiko Furukawa, Satoshi Fukumoto, Masahiko Okada, Heiko Furugen, Hiroshi Miyazaki, Kogo Takamiya, Shinichi Aizawa, Hiroshi Shiku, Toshifumi Matsuyama, Koichi Furukawa

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

T cell development and function in complex ganglioside-lacking (GM2/GD2 synthase gene-disrupted) mice were analyzed. GM1, asialo-GM1, and GD1b were representative gangliosides expressed on T cells of the wild type mice and completely deleted on those of the mutant mice. The sizes and cell numbers of the mutant mice spleen and thymus were significantly reduced. Spleen cells from the mutant mice showed clearly reduced proliferation compared with the wild type when stimulated by interleukin 2 (IL-2) but not when treated with concanavalin A or anti-CD3 cross-linking. Expression levels of IL-2 receptor α, β, and γ, were almost equivalent, and up-regulation of α chain after T cell activation was also similar between the mutant and wild type mice. Activation of JAK1, JAK3, and SAT5 after IL-2 treatment was reduced, and c- fos expression was delayed and reduced in the mutant spleen cells, suggesting that the IL-2 signal was attenuated in the mutant mice probably due to the modulation of IL-2 receptors by the lack of complex gangliosides.

Original languageEnglish
Pages (from-to)13744-13747
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number20
DOIs
Publication statusPublished - May 14 1999
Externally publishedYes

Fingerprint

T-cells
Gangliosides
Interleukin-2
Spleen
Interleukin-2 Receptors
Cells
(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
Chemical activation
Thymus
Concanavalin A
T-Lymphocytes
Genes
Modulation
G(M2) Ganglioside
Thymus Gland
Up-Regulation
Cell Count

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice. / Zhao, Jinmin; Furukawa, Keiko; Fukumoto, Satoshi; Okada, Masahiko; Furugen, Heiko; Miyazaki, Hiroshi; Takamiya, Kogo; Aizawa, Shinichi; Shiku, Hiroshi; Matsuyama, Toshifumi; Furukawa, Koichi.

In: Journal of Biological Chemistry, Vol. 274, No. 20, 14.05.1999, p. 13744-13747.

Research output: Contribution to journalArticle

Zhao, J, Furukawa, K, Fukumoto, S, Okada, M, Furugen, H, Miyazaki, H, Takamiya, K, Aizawa, S, Shiku, H, Matsuyama, T & Furukawa, K 1999, 'Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice', Journal of Biological Chemistry, vol. 274, no. 20, pp. 13744-13747. https://doi.org/10.1074/jbc.274.20.13744
Zhao, Jinmin ; Furukawa, Keiko ; Fukumoto, Satoshi ; Okada, Masahiko ; Furugen, Heiko ; Miyazaki, Hiroshi ; Takamiya, Kogo ; Aizawa, Shinichi ; Shiku, Hiroshi ; Matsuyama, Toshifumi ; Furukawa, Koichi. / Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 20. pp. 13744-13747.
@article{0d7a93ac4053485faf5c6d74f4d52e6b,
title = "Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice",
abstract = "T cell development and function in complex ganglioside-lacking (GM2/GD2 synthase gene-disrupted) mice were analyzed. GM1, asialo-GM1, and GD1b were representative gangliosides expressed on T cells of the wild type mice and completely deleted on those of the mutant mice. The sizes and cell numbers of the mutant mice spleen and thymus were significantly reduced. Spleen cells from the mutant mice showed clearly reduced proliferation compared with the wild type when stimulated by interleukin 2 (IL-2) but not when treated with concanavalin A or anti-CD3 cross-linking. Expression levels of IL-2 receptor α, β, and γ, were almost equivalent, and up-regulation of α chain after T cell activation was also similar between the mutant and wild type mice. Activation of JAK1, JAK3, and SAT5 after IL-2 treatment was reduced, and c- fos expression was delayed and reduced in the mutant spleen cells, suggesting that the IL-2 signal was attenuated in the mutant mice probably due to the modulation of IL-2 receptors by the lack of complex gangliosides.",
author = "Jinmin Zhao and Keiko Furukawa and Satoshi Fukumoto and Masahiko Okada and Heiko Furugen and Hiroshi Miyazaki and Kogo Takamiya and Shinichi Aizawa and Hiroshi Shiku and Toshifumi Matsuyama and Koichi Furukawa",
year = "1999",
month = "5",
day = "14",
doi = "10.1074/jbc.274.20.13744",
language = "English",
volume = "274",
pages = "13744--13747",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "20",

}

TY - JOUR

T1 - Attenuation of interleukin 2 signal in the spleen cells of complex ganglioside-lacking mice

AU - Zhao, Jinmin

AU - Furukawa, Keiko

AU - Fukumoto, Satoshi

AU - Okada, Masahiko

AU - Furugen, Heiko

AU - Miyazaki, Hiroshi

AU - Takamiya, Kogo

AU - Aizawa, Shinichi

AU - Shiku, Hiroshi

AU - Matsuyama, Toshifumi

AU - Furukawa, Koichi

PY - 1999/5/14

Y1 - 1999/5/14

N2 - T cell development and function in complex ganglioside-lacking (GM2/GD2 synthase gene-disrupted) mice were analyzed. GM1, asialo-GM1, and GD1b were representative gangliosides expressed on T cells of the wild type mice and completely deleted on those of the mutant mice. The sizes and cell numbers of the mutant mice spleen and thymus were significantly reduced. Spleen cells from the mutant mice showed clearly reduced proliferation compared with the wild type when stimulated by interleukin 2 (IL-2) but not when treated with concanavalin A or anti-CD3 cross-linking. Expression levels of IL-2 receptor α, β, and γ, were almost equivalent, and up-regulation of α chain after T cell activation was also similar between the mutant and wild type mice. Activation of JAK1, JAK3, and SAT5 after IL-2 treatment was reduced, and c- fos expression was delayed and reduced in the mutant spleen cells, suggesting that the IL-2 signal was attenuated in the mutant mice probably due to the modulation of IL-2 receptors by the lack of complex gangliosides.

AB - T cell development and function in complex ganglioside-lacking (GM2/GD2 synthase gene-disrupted) mice were analyzed. GM1, asialo-GM1, and GD1b were representative gangliosides expressed on T cells of the wild type mice and completely deleted on those of the mutant mice. The sizes and cell numbers of the mutant mice spleen and thymus were significantly reduced. Spleen cells from the mutant mice showed clearly reduced proliferation compared with the wild type when stimulated by interleukin 2 (IL-2) but not when treated with concanavalin A or anti-CD3 cross-linking. Expression levels of IL-2 receptor α, β, and γ, were almost equivalent, and up-regulation of α chain after T cell activation was also similar between the mutant and wild type mice. Activation of JAK1, JAK3, and SAT5 after IL-2 treatment was reduced, and c- fos expression was delayed and reduced in the mutant spleen cells, suggesting that the IL-2 signal was attenuated in the mutant mice probably due to the modulation of IL-2 receptors by the lack of complex gangliosides.

UR - http://www.scopus.com/inward/record.url?scp=0033553506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033553506&partnerID=8YFLogxK

U2 - 10.1074/jbc.274.20.13744

DO - 10.1074/jbc.274.20.13744

M3 - Article

C2 - 10318776

AN - SCOPUS:0033553506

VL - 274

SP - 13744

EP - 13747

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 20

ER -