Attenuation of periostin in retinal müller glia by TNF-α and IFN-γ

Ying Qian Peng, Man Jing Cao, Shigeo Yoshida, Lu Si Zhang, Hui Lan Zeng, Jing Ling Zou, Yoshiyuki Kobayashi, Takahito Nakama, Jing Ming Shi, Song Bai Jia, Ye Di Zhou

Research output: Contribution to journalArticlepeer-review

Abstract

● AIM: To investigate the regulation and mechanisms of periostin expression in retinal Müller glia, and to explore the relevance to retinal neovascularization. ● METHODS: The oxygen-induced retinopathy (OIR) mouse model and the human Moorfield/Institute of Ophthalmology-Müller 1 (MIO-M1) cell line were used in the study. Immunofluorescence staining was used to determine the distribution and expression of periostin and a Müller glial cell marker glutamine synthetase (GS). Cytokines TNF-α and IFN-γ were added to stimulate the MIO-M1 cells. ShRNA was used to knockdown periostin expression in MIO-M1 cells. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was conducted to assess the mRNA expression of periostin. ● RESULTS: Immunofluorescence staining showed that periostin was expressed by MIO-M1 Müller glia. GS-positive Müller glia and periostin increased in OIR retinas, and were partially overlaid. The stimulation of TNF-α and IFN-γ reduced the mRNA expression of periostin significantly and dose-dependently in MIO-M1 cells. Knockdown of periostin reduced mRNA expression of vascular endothelial growth factor A (VEGFA) in MIO-M1 cells, while VEGFA expression was not changed in periostin knock-out OIR retinas. ● CONCLUSION: Müller glia could be one of the main sources of periostin in the retina, and might contribute to the pathogenesis of retinal neovascularization. Proinflammatory cytokines TNF-α and IFN-γ attenuate the periostin expression in retinal Müller glia, which provides a potential and novel method in treating retinal neovascular diseases.

Original languageEnglish
Pages (from-to)212-218
Number of pages7
JournalInternational Journal of Ophthalmology
Volume12
Issue number2
DOIs
Publication statusPublished - Feb 2019

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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