Attenuation of renal ischemia-reperfusion injury by FR167653 in dogs

Hidehisa Kitada, Atsushi Sugitani, Hirofumi Yamamoto, Naoki Otomo, Yasuhiro Okabe, Shigetaka Inoue, Ken Ichi Nishiyama, Takashi Morisaki, Masao Tanaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background. Inflammatory cytokines are known to contribute to ischemia-reperfusion injury. We investigated the effect of FR167653 (FR), a suppressor of interleukin-1β and tumor necrosis factor-α, on ischemia-reperfusion injury of the kidney in dogs. Methods. The left kidney was subjected to ischemia for 60 minutes followed by removal of the right kidney. A control group (n = 10) and an FR group (n = 8) were evaluated for tissue blood flow; resistive index, pulsatility index, arterial oxygen pressure, serum creatinine, blood urea nitrogen, aspartate transaminase, and alanine transaminase levels; interleukin-1β messenger RNA expression in the peripheral blood; apoptotic index; and histopathology. Results. The FR group showed lower creatinine, serum urea nitrogen, aspartate transaminase, and alanine transaminase levels (P <.038 each) and lower interleukin-1β mRNA expression and apoptotic index (P <.041 each) than did the control group. Arterial oxygen pressure during the 120 minutes after reperfusion in the FR group decreased but recovered quickly (P =.024). Renal tissue damage in the FR group was less than that in the control group (P =.036). Conclusions. FR ameliorates ischemia-reperfusion injury of the kidney potentially by reduced production of inflammatory cytokines that may contribute to damage to the ischemic kidney and the distant organs.

Original languageEnglish
Pages (from-to)654-662
Number of pages9
JournalSurgery
Volume131
Issue number6
DOIs
Publication statusPublished - Jan 1 2002

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Kitada, H., Sugitani, A., Yamamoto, H., Otomo, N., Okabe, Y., Inoue, S., ... Tanaka, M. (2002). Attenuation of renal ischemia-reperfusion injury by FR167653 in dogs. Surgery, 131(6), 654-662. https://doi.org/10.1067/msy.2002.124629