Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction

Kazuki Iijima, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this work, we successfully measured a single enzyme reaction in a microfabricated chamber -(510 aL, 7.2 fL, 61 fL and 624 fL) and estimated the effect of enzyme adsorption and denaturation on the reaction rate with inner-surface coated and non-coated chambers. Our results indicate that a single enzyme reaction rate decreased with decreasing the chamber size and that reaction rate in coating chambers slightly increased compared to that in non-coated one.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages854-856
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - Jan 1 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Enzyme activity
Reaction rates
Enzymes
Molecules
Denaturation
Adsorption
Coatings

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Iijima, K., Kaji, N., Okamoto, Y., Tokeshi, M., & Baba, Y. (2009). Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 854-856). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction. / Iijima, Kazuki; Kaji, Noritada; Okamoto, Yukihiro; Tokeshi, Manabu; Baba, Yoshinobu.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 854-856 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Iijima, K, Kaji, N, Okamoto, Y, Tokeshi, M & Baba, Y 2009, Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 854-856, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Iijima K, Kaji N, Okamoto Y, Tokeshi M, Baba Y. Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 854-856. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Iijima, Kazuki ; Kaji, Noritada ; Okamoto, Yukihiro ; Tokeshi, Manabu ; Baba, Yoshinobu. / Atto- and femto-litre microfabrcated structures for investigating depression of enzyme activity at a single molecule reaction. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 854-856 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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