TY - JOUR
T1 - Augmentation of obese (ob) gene expression and leptin secretion in obese spontaneously hypertensive rats (Obese SHR or Koletsky rats)
AU - Hiraoka, Junko
AU - Hosoda, Kiminori
AU - Ogawa, Yoshihiro
AU - Ikeda, Katsumi
AU - Nara, Yasuo
AU - Masuzaki, Hiroaki
AU - Takaya, Kazuhiko
AU - Nakagawa, Kiyoshi
AU - Mashimo, Tomoshi
AU - Sawamura, Makoto
AU - Koletsky, Richard J.
AU - Yamori, Yukio
AU - Nakao, Kazuwa
N1 - Funding Information:
We thank Dr. Yukio Fujisawa and Mr. Toshiyuki Nomura, Takeda Chemical Industries, Ltd. (Osaka, Japan) for discussions. Mr. Ta-kashi Takahashi, Ms. Akiko Kitamura, and Mr. Masanobu Taka-hashi are also acknowledged for help in tissue sampling. We also thank Ms. Kaori Hiramatsu for her excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare, Japan Diabetes Foundation, and Uehara Memorial Foundation, and by a grant for diabetes research from Otsuka Pharmaceutical Co., Ltd. (Tokushima, Japan).
PY - 1997/2/24
Y1 - 1997/2/24
N2 - To explore the pathophysiologic roles of the obese (ob) gene product, leptin, in the development of obesity and hypertension, we examined ob gene expression and leptin secretion in obese spontaneously hypertensive rats (obese SHR or Koletsky rats) at the stage of established obesity and hypertension. Expression of the ob gene was augmented in the epididymal, mesenteric, subcutaneous, and retroperitoneal white adipose tissue (WAT) from 20-week-old male obese SHR compared to their lean littermates (lean SHR). Using a radioimmunoassay for rat leptin, we also measured plasma leptin levels in 20-week-old lean and obese SHR. Plasma leptin levels in obese SHR (292.5 ± 37.1 ng/ml) were more than 100-fold higher than those in lean SHR (2.8 ± 1.0 ng/ml). The present study demonstrates that ob gene expression and leptin secretion are markedly augmented in obese SHR.
AB - To explore the pathophysiologic roles of the obese (ob) gene product, leptin, in the development of obesity and hypertension, we examined ob gene expression and leptin secretion in obese spontaneously hypertensive rats (obese SHR or Koletsky rats) at the stage of established obesity and hypertension. Expression of the ob gene was augmented in the epididymal, mesenteric, subcutaneous, and retroperitoneal white adipose tissue (WAT) from 20-week-old male obese SHR compared to their lean littermates (lean SHR). Using a radioimmunoassay for rat leptin, we also measured plasma leptin levels in 20-week-old lean and obese SHR. Plasma leptin levels in obese SHR (292.5 ± 37.1 ng/ml) were more than 100-fold higher than those in lean SHR (2.8 ± 1.0 ng/ml). The present study demonstrates that ob gene expression and leptin secretion are markedly augmented in obese SHR.
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U2 - 10.1006/bbrc.1997.6145
DO - 10.1006/bbrc.1997.6145
M3 - Article
C2 - 9070850
AN - SCOPUS:0031584837
SN - 0006-291X
VL - 231
SP - 582
EP - 585
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
