Augmentation of the resistance against listeria monocytogenes by oral administration of a hot water extt of chlorella vulgarism mice

Takashi Hasegawa, Masao Okuda, Kikuo Nomoto, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Oral administration of a hot water extract of Chlorella vulgaris(CVE)(20mg/mouse, 10 consecutive days) augmented the resistance against an i.p. infection with Listeria monocytogenes in mice. The numbers of bacteria in a CVE-administered group were significantly lower in the peritoneal cavity or spleen than those in a control group. FCM analysis revealed that γdelta;±Thy 1.2± cells in the nonadherent PEC from CVE-administered mice increased more prominently in number at the early stage on day 3 or on day 5 after infection as compared with those in control mice. The increment of γdelta;±Thy 1.2± T cells was also evident in spleen in CVE-administered mice at this stage after infection. The proportion of TCRαbeta;plusmn;Thy 1.2± T cells in the nonadherent PEC of a control group increased from 13% on day 0 to 49% at the late stage on day 10 after infection, whereas the proportion of TCRαbeta;±Thy 1.2± T cells in the nonadherent PEC in CVE-administered mice increased to 64% on this stage after infection in association with augmentation of DTH response to Listeria. These results suggest that CVE-administration effectively augment cell-mediated immunity against Listeria through the increment of γdelta;± T cells in the early phase and the increment of αbeta;± T cells in the late phase after listerial infection.

    Original languageEnglish
    Pages (from-to)191-202
    Number of pages12
    JournalImmunopharmacology and Immunotoxicology
    Volume16
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 1994

    Fingerprint

    Listeria
    Chlorella
    T-cells
    Listeria monocytogenes
    Oral Administration
    Water
    T-Lymphocytes
    Infection
    Spleen
    Chlorella vulgaris
    Control Groups
    Peritoneal Cavity
    Bacteria
    Cellular Immunity

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology
    • Toxicology
    • Pharmacology

    Cite this

    Augmentation of the resistance against listeria monocytogenes by oral administration of a hot water extt of chlorella vulgarism mice. / Hasegawa, Takashi; Okuda, Masao; Nomoto, Kikuo; Yoshikai, Yasunobu.

    In: Immunopharmacology and Immunotoxicology, Vol. 16, No. 2, 01.01.1994, p. 191-202.

    Research output: Contribution to journalArticle

    Hasegawa, Takashi ; Okuda, Masao ; Nomoto, Kikuo ; Yoshikai, Yasunobu. / Augmentation of the resistance against listeria monocytogenes by oral administration of a hot water extt of chlorella vulgarism mice. In: Immunopharmacology and Immunotoxicology. 1994 ; Vol. 16, No. 2. pp. 191-202.
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