Augmented expression of the obese gene in the adipose tissue from rats fed high-fat diet

Hiroaki Masuzaki, Yoshihiro Ogawa, Kiminori Hosoda, Teruo Kawada, Toru Fushiki, Kazuwa Nakao

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Expression of the obese (ob) gene is augmented in the adipose tissue in several rodent models of genetic obesity. In the present study, we examined the ob gene expression in a rodent model of acquired obesity obtained by pure overfeeding of normal rats. Male Sprague-Dawley rats at 8 weeks of age were fed standard diet or high-fat diet. Rats fed high-fat diet developed moderate degree of obesity, hyperglycemia, and hyperlipidemia as compared with rats fed standard diet. Northern blot analysis revealed that the ob gene is expressed abundantly in the adipose tissue obtained from the epididymal, mesenteric, subcutaneous, retroperitoneal, and interscapular fat pads in rats fed standard diet. Expression of the ob gene was augmented in all the adipose tissue examined in rats fed high-fat diet. The present study demonstrates that the ob gene expression is augmented in the adipose tissue in diet-induced obesity, thereby suggesting the pathophysiologic roles of the ob gene in acquired obesity.

Original languageEnglish
Pages (from-to)355-358
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume216
Issue number1
DOIs
Publication statusPublished - Nov 2 1995

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High Fat Diet
Nutrition
Adipose Tissue
Rats
Obesity
Genes
Fats
Tissue
Gene Expression
Diet
Rodentia
Gene expression
Intra-Abdominal Fat
Genetic Models
Hyperlipidemias
Hyperglycemia
Northern Blotting
Sprague Dawley Rats

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Augmented expression of the obese gene in the adipose tissue from rats fed high-fat diet. / Masuzaki, Hiroaki; Ogawa, Yoshihiro; Hosoda, Kiminori; Kawada, Teruo; Fushiki, Toru; Nakao, Kazuwa.

In: Biochemical and Biophysical Research Communications, Vol. 216, No. 1, 02.11.1995, p. 355-358.

Research output: Contribution to journalArticle

Masuzaki, Hiroaki ; Ogawa, Yoshihiro ; Hosoda, Kiminori ; Kawada, Teruo ; Fushiki, Toru ; Nakao, Kazuwa. / Augmented expression of the obese gene in the adipose tissue from rats fed high-fat diet. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 216, No. 1. pp. 355-358.
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