Automated chemiluminescence immunoassay for a nonionic surfactant using a recycled spinning-pausing controlled washing procedure on a compact disc-type microfluidic platform

Shuai Guo, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    A fully automated and integrated chemiluminescence immunoassay, carried out on a compact disc (CD)-type microfluidic platform, for the detection of alkylphenol polyethoxylates (APnEOs) is described. The pattern of the CD-type microchip was designed so as to permit the sequential solution delivery of the sample solution, the washing solution and the luminol solution, which are required in the chemiluminescence immunoassay process, along with a designed rotation program for spinning the CD-type microchip. The procedure for flowing the washing solution, the volume of which was limited on the CD-type microchip, was optimized by using a recycled spinning-pausing rotation program to overcome the non-specific adsorption of the horseradish peroxidase labeled APnEOs at the detection area. The detection limit of the immunoassay is about 10 ppb.

    Original languageEnglish
    Pages (from-to)100-106
    Number of pages7
    JournalTalanta
    Volume133
    DOIs
    Publication statusPublished - Feb 2015

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

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