Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion

Shinichi Kawano, takehiro torisu, Motohiro Esaki, kumiko torisu, Yuichi Matsuno, Takanari Kitazono

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Adhesion of cells to the extracellular matrix (ECM) via focal adhesions (FAs) is crucial for cell survival, migration, and differentiation. Although the regulation of FAs, including by integrins and the ECM, is important to cell behavior, how FAs are regulated is not well known. Autophagy is induced by both cell adhesion and cell detachment. Here, we showed that autophagosomes are located close to internalized collagen and paxillin, which is a well-known marker of FAs. Autophagy-deficient cells showed increased levels of internalized collagen compared with control cells. Moreover, paxillin exhibited a more peripheral distribution and the area of paxillin was increased, and adhesion-induced focal adhesion kinase signaling was impaired and adhesion was enhanced, in autophagy-deficient cells. These results suggest that autophagy suppressed cell adhesion by regulating internalized ECM and FAs.

Original languageEnglish
Pages (from-to)1644-1653
Number of pages10
JournalBiology Open
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 15 2017

Fingerprint

Focal Adhesions
autophagy
Cell adhesion
Autophagy
cell adhesion
Cell Adhesion
adhesion
collagen
Paxillin
Collagen
Adhesion
Degradation
degradation
Extracellular Matrix
extracellular matrix
cells
Focal Adhesion Protein-Tyrosine Kinases
non-specific protein-tyrosine kinase
Integrins
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion. / Kawano, Shinichi; torisu, takehiro; Esaki, Motohiro; torisu, kumiko; Matsuno, Yuichi; Kitazono, Takanari.

In: Biology Open, Vol. 6, No. 11, 15.11.2017, p. 1644-1653.

Research output: Contribution to journalArticle

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