Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma

Mamoru Harada, Goro Matsuzaki, Yasunobu Yoshikai, Noritada Kobayashi, Shin Kurosawa, Hiroaki Takimoto, Kikuo Nomoto

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    A CD4+ heat shock protein (hsp) 60-recognizing autoreactive T-ceil line (BASL1) and clone (BASL1.1) were examined for their antitumor activity against major histocompatibility complex class II” syngeneic Meth A fibrosarcoma (Meth A), which was immunofluorescently stained with monoclonal antibody specific for hsp 60. In in vitro proliferative assay, BASL1.1 was suggested to recognize Meth A-derived hsp 60 presented by syngeneic antigen-presenting cells in a major histocompatibility complex class II-restricted manner. This cell line and clone showed antitumor activity in tumor-neutralizing (Winn) assay. BASL1 and BASL1.1 cells produced y-interferon, tumor necrosis factor, and interleukin 2 but not interleukin 4 by the stimulation with syngeneic spleen cells. In cytolytic assay, these cell lines and clones showed neither direct nor indirect (bystander) cytol-ysis against Meth A. In cytostatic assay, these cells inhibited the proliferation of Meth A in the presence of syngeneic macrophages, and this activity was abrogated by the addition of anti-y-interferon monoclonal antibody. Recombinant γ-interferon could induce cytostatic activity only in the presence of macrophages, and tumor necrosis factor synergized this activity. Antitumor activity induced by BASL1 was abrogated by the administration of anti-CD8 monoclonal antibody in vivo, suggesting that CD8+ cytotoxic T-lymphocytes are essential and final effector cells for BASLl-mediated Meth A rejection. These findings indicate that CD4+ autoreactive and hsp 60-recognizing T-cells show two types of antitumor activity: cytostasis and induction of tumor-specific cytotoxic T-lymphocytes. Furthermore, these results imply that tumor-specific immunity could be elicited by CD4+ helper T-cells which recognize hsp.

    Original languageEnglish
    Pages (from-to)106-111
    Number of pages6
    JournalCancer Research
    Volume53
    Issue number1
    Publication statusPublished - Jan 1 1993

    Fingerprint

    Chaperonin 60
    Fibrosarcoma
    Interferons
    T-Lymphocytes
    Clone Cells
    Monoclonal Antibodies
    Cytostatic Agents
    Cytotoxic T-Lymphocytes
    Major Histocompatibility Complex
    Tumor Necrosis Factor-alpha
    Macrophages
    Cell Line
    Neoplasms
    Antigen-Presenting Cells
    Heat-Shock Proteins
    Helper-Inducer T-Lymphocytes
    Interleukin-4
    Interleukin-2
    Immunity
    Spleen

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

    Cite this

    Harada, M., Matsuzaki, G., Yoshikai, Y., Kobayashi, N., Kurosawa, S., Takimoto, H., & Nomoto, K. (1993). Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma. Cancer Research, 53(1), 106-111.

    Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma. / Harada, Mamoru; Matsuzaki, Goro; Yoshikai, Yasunobu; Kobayashi, Noritada; Kurosawa, Shin; Takimoto, Hiroaki; Nomoto, Kikuo.

    In: Cancer Research, Vol. 53, No. 1, 01.01.1993, p. 106-111.

    Research output: Contribution to journalArticle

    Harada, M, Matsuzaki, G, Yoshikai, Y, Kobayashi, N, Kurosawa, S, Takimoto, H & Nomoto, K 1993, 'Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma', Cancer Research, vol. 53, no. 1, pp. 106-111.
    Harada M, Matsuzaki G, Yoshikai Y, Kobayashi N, Kurosawa S, Takimoto H et al. Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma. Cancer Research. 1993 Jan 1;53(1):106-111.
    Harada, Mamoru ; Matsuzaki, Goro ; Yoshikai, Yasunobu ; Kobayashi, Noritada ; Kurosawa, Shin ; Takimoto, Hiroaki ; Nomoto, Kikuo. / Autoreactive and Heat Shock Protein 60-recognizing CD4+ T-Cells Show Antitumor Activity against Syngeneic Fibrosarcoma. In: Cancer Research. 1993 ; Vol. 53, No. 1. pp. 106-111.
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    abstract = "A CD4+ heat shock protein (hsp) 60-recognizing autoreactive T-ceil line (BASL1) and clone (BASL1.1) were examined for their antitumor activity against major histocompatibility complex class II” syngeneic Meth A fibrosarcoma (Meth A), which was immunofluorescently stained with monoclonal antibody specific for hsp 60. In in vitro proliferative assay, BASL1.1 was suggested to recognize Meth A-derived hsp 60 presented by syngeneic antigen-presenting cells in a major histocompatibility complex class II-restricted manner. This cell line and clone showed antitumor activity in tumor-neutralizing (Winn) assay. BASL1 and BASL1.1 cells produced y-interferon, tumor necrosis factor, and interleukin 2 but not interleukin 4 by the stimulation with syngeneic spleen cells. In cytolytic assay, these cell lines and clones showed neither direct nor indirect (bystander) cytol-ysis against Meth A. In cytostatic assay, these cells inhibited the proliferation of Meth A in the presence of syngeneic macrophages, and this activity was abrogated by the addition of anti-y-interferon monoclonal antibody. Recombinant γ-interferon could induce cytostatic activity only in the presence of macrophages, and tumor necrosis factor synergized this activity. Antitumor activity induced by BASL1 was abrogated by the administration of anti-CD8 monoclonal antibody in vivo, suggesting that CD8+ cytotoxic T-lymphocytes are essential and final effector cells for BASLl-mediated Meth A rejection. These findings indicate that CD4+ autoreactive and hsp 60-recognizing T-cells show two types of antitumor activity: cytostasis and induction of tumor-specific cytotoxic T-lymphocytes. Furthermore, these results imply that tumor-specific immunity could be elicited by CD4+ helper T-cells which recognize hsp.",
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