TY - JOUR
T1 - Autoreactive T cells from normal mice recognize mycobacterial 65 kd heat-shock protein from mycobacterium bovis
AU - Matsuzaki, Goro
AU - Yoshikal, Yasunobu
AU - Harada, Mamoru
AU - Nomoto, Kikuo
N1 - Funding Information:
We thank Dr Ralph Kubo for providing the anti-TCR 0-chain mAb (H57-597), Dr Hans Hengartner for providing the anti-TCR Vg6 mAb (44-22-1), Dr Uwe D., Staerz for providing the ant>TCR Vfl8 mAb (F23.1), Dr Ruurd van der Zee for providing recombinant 65 kd nsp of M. bovis, and Mr B Quinn for comments on our manuscript This work was supported in part by grants to Y. Yoshikai from the Ministry of Education, Science and Culture (6240167) (01015081) and received financial support from the UNDP/Worid Bank/WHO Special Program for Research and Training in Tropical Diseases (TOR).
PY - 1991/2
Y1 - 1991/2
N2 - We established a TCR Vβ6+ CD4+ autoreactive T cell line from lymph nodecells of unprimed BALB/c mice bred in a specific pathogen-free condition. The T cell line proliferated In the presenceof Irradiated spleen cells expressing MHC class II I-Ad and/or I-Ed molecules. The proliferatlveresponse of the autoreactive T cell line was significantly enhanced In response to 65 kd heat-shock protein (hsp) from Mycobacterium bovis, a member of the hsp60 family, which is highly conserved in both prokaryotes and eukaryotes. Our results suggest that the autoreactive T cells in normal mice may recognize endogenous peptldes of self-hsp60 bound to MHC class IIgene products and are cross-reactive to mycobacterial 65 kd hsp. The autoreactive hsp-specific T cells may therefore play an important role in the immune surveillance against invasion of various pathogens and tumors by recognizing Infected and transformed autoiogous cells that express high levels of endogenous hsp60.
AB - We established a TCR Vβ6+ CD4+ autoreactive T cell line from lymph nodecells of unprimed BALB/c mice bred in a specific pathogen-free condition. The T cell line proliferated In the presenceof Irradiated spleen cells expressing MHC class II I-Ad and/or I-Ed molecules. The proliferatlveresponse of the autoreactive T cell line was significantly enhanced In response to 65 kd heat-shock protein (hsp) from Mycobacterium bovis, a member of the hsp60 family, which is highly conserved in both prokaryotes and eukaryotes. Our results suggest that the autoreactive T cells in normal mice may recognize endogenous peptldes of self-hsp60 bound to MHC class IIgene products and are cross-reactive to mycobacterial 65 kd hsp. The autoreactive hsp-specific T cells may therefore play an important role in the immune surveillance against invasion of various pathogens and tumors by recognizing Infected and transformed autoiogous cells that express high levels of endogenous hsp60.
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U2 - 10.1093/intimm/3.2.215
DO - 10.1093/intimm/3.2.215
M3 - Article
C2 - 1709051
AN - SCOPUS:0026028316
VL - 3
SP - 215
EP - 220
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 2
ER -