TY - JOUR
T1 - Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe
AU - Chardwiriyapreecha, Soracom
AU - Mukaiyama, Hiroyuki
AU - Sekito, Takayuki
AU - Iwaki, Tomoko
AU - Takegawa, Kaoru
AU - Kakinuma, Yoshimi
N1 - Funding Information:
We thank Shuichi Katoh and Shiro Kajiwara for technical assistance and Dr. Taro Nakamura for providing plasmid pTN197. This work was supported in part by a Grant-in-Aid for Scientific Research (to Y.K.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan .
PY - 2010/6
Y1 - 2010/6
N2 - We identified SPBC1685.07c of Schizosaccharomyces pombe as a novel vacuolar protein, Avt5p, with similarity to vacuolar amino acid transporters Avt5p from Saccharomyces cerevisiae. Avt5p localizes to the vacuolar membrane and upon disruption of avt5, uptake of histidine, glutamate, tyrosine, arginine, lysine or serine was impaired. During nitrogen starvation, the transient increase of vacuolar lysine transport observed for wild-type cells still occurred in the mutant cells, however, uptake of glutamate did not significantly increase in response to nitrogen starvation. Our results show that under diverse growth conditions Avt5p is involved in vacuolar transport of a selective set of amino acids.
AB - We identified SPBC1685.07c of Schizosaccharomyces pombe as a novel vacuolar protein, Avt5p, with similarity to vacuolar amino acid transporters Avt5p from Saccharomyces cerevisiae. Avt5p localizes to the vacuolar membrane and upon disruption of avt5, uptake of histidine, glutamate, tyrosine, arginine, lysine or serine was impaired. During nitrogen starvation, the transient increase of vacuolar lysine transport observed for wild-type cells still occurred in the mutant cells, however, uptake of glutamate did not significantly increase in response to nitrogen starvation. Our results show that under diverse growth conditions Avt5p is involved in vacuolar transport of a selective set of amino acids.
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U2 - 10.1016/j.febslet.2010.04.012
DO - 10.1016/j.febslet.2010.04.012
M3 - Article
C2 - 20388511
AN - SCOPUS:77952952457
SN - 0014-5793
VL - 584
SP - 2339
EP - 2345
JO - FEBS Letters
JF - FEBS Letters
IS - 11
ER -