Abstract
We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin- dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video- enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 μM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 μM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 μM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.
| Original language | English |
|---|---|
| Pages (from-to) | 135-138 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 826 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 24 1999 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Cite this
Axonal transport is inhibited by a protein kinase C inhibitor in cultured isolated mouse dorsal root ganglion cells. / Hiruma, Hiromi; Maruyama, Hiroshi; Katakura, Takashi; Simada, Zyun'ici B.; Nishida, Sanae; Hoka, Sumio; Takenaka, Toshifumi; Kawakami, Tadashi.
In: Brain Research, Vol. 826, No. 1, 24.04.1999, p. 135-138.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Axonal transport is inhibited by a protein kinase C inhibitor in cultured isolated mouse dorsal root ganglion cells
AU - Hiruma, Hiromi
AU - Maruyama, Hiroshi
AU - Katakura, Takashi
AU - Simada, Zyun'ici B.
AU - Nishida, Sanae
AU - Hoka, Sumio
AU - Takenaka, Toshifumi
AU - Kawakami, Tadashi
PY - 1999/4/24
Y1 - 1999/4/24
N2 - We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin- dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video- enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 μM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 μM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 μM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.
AB - We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin- dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video- enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 μM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 μM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 μM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.
UR - http://www.scopus.com/inward/record.url?scp=0033599706&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033599706&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(99)01249-4
DO - 10.1016/S0006-8993(99)01249-4
M3 - Article
C2 - 10216205
AN - SCOPUS:0033599706
VL - 826
SP - 135
EP - 138
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -