TY - JOUR
T1 - Axonal transport is inhibited by a protein kinase C inhibitor in cultured isolated mouse dorsal root ganglion cells
AU - Hiruma, Hiromi
AU - Maruyama, Hiroshi
AU - Katakura, Takashi
AU - Simada, Zyun'ici B.
AU - Nishida, Sanae
AU - Hoka, Sumio
AU - Takenaka, Toshifumi
AU - Kawakami, Tadashi
N1 - Funding Information:
This work was partly supported by the Academic Frontier Project of the Ministry of Education, Science, Sports and Culture, Japan.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/4/24
Y1 - 1999/4/24
N2 - We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin- dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video- enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 μM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 μM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 μM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.
AB - We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin- dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video- enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 μM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 μM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 μM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.
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U2 - 10.1016/S0006-8993(99)01249-4
DO - 10.1016/S0006-8993(99)01249-4
M3 - Article
C2 - 10216205
AN - SCOPUS:0033599706
VL - 826
SP - 135
EP - 138
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -