Axonal transport is inhibited by a protein kinase C inhibitor in cultured isolated mouse dorsal root ganglion cells

Hiromi Hiruma, Hiroshi Maruyama, Takashi Katakura, Zyun'ici B. Simada, Sanae Nishida, Sumio Hoka, Toshifumi Takenaka, Tadashi Kawakami

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Abstract

We investigated roles of protein kinase C (PKC) and Ca2+/calmodulin- dependent protein II (CAM II) kinase activities in the maintenance of axonal transport in cultured isolated mouse dorsal root ganglion (DRG) cells. Video- enhanced microscopic recordings revealed that the PKC inhibitor chelerythrine (1 μM) reduced anterograde and retrograde axonal transport, while the CAM II kinase inhibitor KN-62 (10 μM) had no effect. Morphological observation showed that neurite growth was prevented by the presence of chelerythrine (1 μM). From these results, we conclude that PKC activity is required to maintain axonal transport and thereby neurite growth.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalBrain Research
Volume826
Issue number1
DOIs
Publication statusPublished - Apr 24 1999

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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    Hiruma, H., Maruyama, H., Katakura, T., Simada, Z. B., Nishida, S., Hoka, S., Takenaka, T., & Kawakami, T. (1999). Axonal transport is inhibited by a protein kinase C inhibitor in cultured isolated mouse dorsal root ganglion cells. Brain Research, 826(1), 135-138. https://doi.org/10.1016/S0006-8993(99)01249-4