B cell potential can be obtained from pre-circulatory yolk sac, but with low frequency

Daisuke Sugiyama, Minetaro Ogawa, Kazuki Nakao, Noriko Osumi, Satomi Nishikawa, Shin ichi Nishikawa, Ken ichi Arai, Tatsutoshi Nakahata, Kohichiro Tsuji

Research output: Contribution to journalArticle

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Abstract

The ontogenic source of definitive hematopoietic system has been identified in non-mammalian vertebrates such as birds and amphibians by orthotopic embryo grafting, but remains unclear for mammals because of technical difficulties. Here, we successfully generated mouse chimeras by grafting yolk sac (YS) on YS of the host embryos before establishing circulation between YS and embryo proper and cultured the whole embryo for 66 h. Donor YS were isolated from C57BL/6 Ly-5.1 and EGFP-transgenic mouse embryos, and recipient embryos from C57BL/6 Ly-5.2 mouse. Almost one-half of the grafts in YS-YS chimeras survived and had obvious blood flow; graft-derived cells comprised 12.7 ± 0.9% of the blood cells in the circulation. These graft-derived blood cells consisted mainly of erythroid cells, some myeloid cells and a few blastic cells. In addition, CD19+ B cells were generated from the graft-derived cells isolated from aorta-gonad-mesonephros (AGM) regions of the YS-YS chimeras; however, the frequency of the YS-derived B cell was low (1.0 ± 0.6%) when co-cultured with OP9 stromal cells. These results demonstrate that B cell potential exists in YS before the circulation. Although the major source for B cell is intra-embryonic AGM region, YS may contribute to definitive lymphopoiesis in vivo in mice.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalDevelopmental Biology
Volume301
Issue number1
DOIs
Publication statusPublished - Jan 1 2007
Externally publishedYes

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Yolk Sac
B-Lymphocytes
Embryonic Structures
Mesonephros
Transplants
Gonads
Aorta
Blood Cells
Lymphopoiesis
Hematopoietic System
Erythroid Cells
Amphibians
Myeloid Cells
Stromal Cells
Transgenic Mice
Birds
Vertebrates
Mammals

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Sugiyama, D., Ogawa, M., Nakao, K., Osumi, N., Nishikawa, S., Nishikawa, S. I., ... Tsuji, K. (2007). B cell potential can be obtained from pre-circulatory yolk sac, but with low frequency. Developmental Biology, 301(1), 53-61. https://doi.org/10.1016/j.ydbio.2006.09.042

B cell potential can be obtained from pre-circulatory yolk sac, but with low frequency. / Sugiyama, Daisuke; Ogawa, Minetaro; Nakao, Kazuki; Osumi, Noriko; Nishikawa, Satomi; Nishikawa, Shin ichi; Arai, Ken ichi; Nakahata, Tatsutoshi; Tsuji, Kohichiro.

In: Developmental Biology, Vol. 301, No. 1, 01.01.2007, p. 53-61.

Research output: Contribution to journalArticle

Sugiyama, D, Ogawa, M, Nakao, K, Osumi, N, Nishikawa, S, Nishikawa, SI, Arai, KI, Nakahata, T & Tsuji, K 2007, 'B cell potential can be obtained from pre-circulatory yolk sac, but with low frequency', Developmental Biology, vol. 301, no. 1, pp. 53-61. https://doi.org/10.1016/j.ydbio.2006.09.042
Sugiyama, Daisuke ; Ogawa, Minetaro ; Nakao, Kazuki ; Osumi, Noriko ; Nishikawa, Satomi ; Nishikawa, Shin ichi ; Arai, Ken ichi ; Nakahata, Tatsutoshi ; Tsuji, Kohichiro. / B cell potential can be obtained from pre-circulatory yolk sac, but with low frequency. In: Developmental Biology. 2007 ; Vol. 301, No. 1. pp. 53-61.
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