b-series Ganglioside deficiency exhibits no definite changes in the neurogenesis and the sensitivity to Fas-mediated apoptosis but impairs regeneration of the lesioned hypoglossal nerve

Masahiko Okada, Michi Ichiro Itoh, Masashi Haraguchi, Tetsuya Okajima, Masahiro Inoue, Hideto Oishi, Yoichi Matsuda, Tsutomu Iwamoto, Toshihiro Kawano, Satoshi Fukumoto, Hiroshi Miyazaki, Keiko Furukawa, Shinichi Aizawa, Koichi Furukawa

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

The polymorphic carbohydrate structures of gangliosides play regulatory roles. In particular, b-series gangliosides, all of which contain α-2,8 sialic acids, have been considered to be critical in various biological events such as adhesion, toxin binding, neurite extension, cell growth, and apoptosis. To clarify the physiological functions of b-series gangliosides in vivo, we have established a gene knockout mouse of GD3 synthase. Although all b-series structures were deleted in the mutant mice, they showed an almost complete nervous tissue morphology with no apparent abnormal behavior. Moreover, no differences in Fas-mediated apoptotic reaction of lymphocytes between wild type and the mutant mice were detected. However, the mutant mice exhibited clearly reduced regeneration of axotomized hypoglossal nerves compared with the wild type, suggesting that b-series gangliosides are more important in the repair rather than in the differentiation of the nervous system and apoptotic process induced via Fas.

Original languageEnglish
Pages (from-to)1633-1636
Number of pages4
JournalJournal of Biological Chemistry
Volume277
Issue number3
DOIs
Publication statusPublished - Jan 18 2002

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Hypoglossal Nerve
Gangliosides
Neurogenesis
Regeneration
Apoptosis
Sialic Acids
Nerve Tissue
Gene Knockout Techniques
Lymphocytes
Cell growth
Neurology
Neurites
Knockout Mice
Nervous System
Repair
Adhesion
Genes
Carbohydrates
Tissue
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

b-series Ganglioside deficiency exhibits no definite changes in the neurogenesis and the sensitivity to Fas-mediated apoptosis but impairs regeneration of the lesioned hypoglossal nerve. / Okada, Masahiko; Itoh, Michi Ichiro; Haraguchi, Masashi; Okajima, Tetsuya; Inoue, Masahiro; Oishi, Hideto; Matsuda, Yoichi; Iwamoto, Tsutomu; Kawano, Toshihiro; Fukumoto, Satoshi; Miyazaki, Hiroshi; Furukawa, Keiko; Aizawa, Shinichi; Furukawa, Koichi.

In: Journal of Biological Chemistry, Vol. 277, No. 3, 18.01.2002, p. 1633-1636.

Research output: Contribution to journalArticle

Okada, M, Itoh, MI, Haraguchi, M, Okajima, T, Inoue, M, Oishi, H, Matsuda, Y, Iwamoto, T, Kawano, T, Fukumoto, S, Miyazaki, H, Furukawa, K, Aizawa, S & Furukawa, K 2002, 'b-series Ganglioside deficiency exhibits no definite changes in the neurogenesis and the sensitivity to Fas-mediated apoptosis but impairs regeneration of the lesioned hypoglossal nerve', Journal of Biological Chemistry, vol. 277, no. 3, pp. 1633-1636. https://doi.org/10.1074/jbc.C100395200
Okada, Masahiko ; Itoh, Michi Ichiro ; Haraguchi, Masashi ; Okajima, Tetsuya ; Inoue, Masahiro ; Oishi, Hideto ; Matsuda, Yoichi ; Iwamoto, Tsutomu ; Kawano, Toshihiro ; Fukumoto, Satoshi ; Miyazaki, Hiroshi ; Furukawa, Keiko ; Aizawa, Shinichi ; Furukawa, Koichi. / b-series Ganglioside deficiency exhibits no definite changes in the neurogenesis and the sensitivity to Fas-mediated apoptosis but impairs regeneration of the lesioned hypoglossal nerve. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 3. pp. 1633-1636.
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