B-Z DNA transition triggered by a cationic comb-type copolymer

Naohiko Shimada, Arihiro Kano, Atsushi Maruyama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The conformational transition from right-handed B-DNA to left-handed Z-DNA - the B-Z transition - has received increased attention recently because of its potential roles in biological systems and its applicability to bionanotechnology. Though the B-Z transition of poly(dG-dC) · poiy(dG-dC) is inducible under high salt concentration conditions (over 4 M NaCl) or by addition of multivalent cations, such as hexaamminecobalt(III), no cationic polymer were known to induce the transition. In this study, it is shown by circular dichroism and UV spectroscopy that the cationic comb-type copolymer, poly(L-lysine)-graft-dextran, but not poly(L-lysine) homopolymer or a basic peptide, induces the B-Z transition of poly(dG-dC) · poly(dG-dC). At a cationic amino group concentration of 10-4M the copolymer stabilizes Z-DNA. The transition pathway from the B to the Z form is different to that observed previously. We speculate that the cationic backbone of the copolymer, which reduces electrostatic repulsion among DNA phosphate groups, and the hydrophilic dextran chains, which reduce activity of water, cooperate to induce the B-Z transition. The copolymer specifically modified the micro-environment around DNA molecules to induce Z-DNA formation through stable and spontaneous inter-polyelectrolyte complex formation

Original languageEnglish
Pages (from-to)3590-3595
Number of pages6
JournalAdvanced Functional Materials
Volume19
Issue number22
DOIs
Publication statusPublished - Nov 23 2009

Fingerprint

Z-Form DNA
copolymers
DNA
Copolymers
deoxyribonucleic acid
B-Form DNA
Circular dichroism spectroscopy
Polylysine
Dextran
Biological systems
dextrans
Dextrans
Polyelectrolytes
Ultraviolet spectroscopy
lysine
Cations
Electrostatics
Polymers
Salts
Phosphates

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

B-Z DNA transition triggered by a cationic comb-type copolymer. / Shimada, Naohiko; Kano, Arihiro; Maruyama, Atsushi.

In: Advanced Functional Materials, Vol. 19, No. 22, 23.11.2009, p. 3590-3595.

Research output: Contribution to journalArticle

Shimada, Naohiko ; Kano, Arihiro ; Maruyama, Atsushi. / B-Z DNA transition triggered by a cationic comb-type copolymer. In: Advanced Functional Materials. 2009 ; Vol. 19, No. 22. pp. 3590-3595.
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