Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation

Miho Hayashi, Yoshitoshi Ogura, Elizabeth J. Harry, Naotake Ogasawara, Shigeki Moriya

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalFEMS microbiology letters
Volume247
Issue number1
DOIs
Publication statusPublished - Jun 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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