Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch

Mohd Huzairi Mohd Zainudin, Mohd Ali Hassan, Umi Kalsom Md Shah, Norhani Abdullah, Mitsunori Tokura, Hisashi Yasueda, Yoshihito Shirai, Kenji Sakai, Azhari Samsu Baharuddin

Research output: Contribution to journalReview article

8 Citations (Scopus)

Abstract

Bacterial community structure and biochemical changes during the composting of lignocellulosic oil palm empty bunch (EFB) and palm oil mill effluent (POME) anaerobic sludge were studied by examining the succession of the bacterial community and its association with changes in lignocellulosic components by denaturing gradient gel electrophoresis (DGGE) and the 16S rRNA gene clone library. During composting, a major reduction in cellulose after 10 days from 50% to 19% and the carbon content from 44% to 27% towards the end of the 40-day composting period were observed. The C/N ratio also decreased. A drastic change in the bacterial community structure and diversity throughout the composting process was clearly observed using PCR-DGGE banding patterns. The bacterial community drastically shifted between the thermophilic and maturing stages. 16s rRNA clones belonging to the genera Bacillus, Exiguobacterium, Desemzia, and Planococcus were the dominant groups throughout composting. The species closely related to Solibacillus silvestris were found to be major contributors to changes in the lignocellulosic component. Clones identified as Thermobacillus xylanilyticus, Brachybacterium faecium, Cellulosimicrobium cellulans, Cellulomonas sp., and Thermobifida fusca, which are known to be lignocellulosic-degrading bacteria, were also detected and are believed to support the lignocellulose degradation.

Original languageEnglish
Pages (from-to)316-335
Number of pages20
JournalBioResources
Volume9
Issue number1
Publication statusPublished - Jan 23 2014

Fingerprint

Composting
Palm oil
Fruits
composting
community structure
fruit
oil
clone
Electrophoresis
electrokinesis
Gels
gel
Sewage sludge
Bacilli
Cellulose
cellulose
Effluents
Bacteria
mill
Carbon

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Zainudin, M. H. M., Hassan, M. A., Md Shah, U. K., Abdullah, N., Tokura, M., Yasueda, H., ... Baharuddin, A. S. (2014). Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch. BioResources, 9(1), 316-335.

Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch. / Zainudin, Mohd Huzairi Mohd; Hassan, Mohd Ali; Md Shah, Umi Kalsom; Abdullah, Norhani; Tokura, Mitsunori; Yasueda, Hisashi; Shirai, Yoshihito; Sakai, Kenji; Baharuddin, Azhari Samsu.

In: BioResources, Vol. 9, No. 1, 23.01.2014, p. 316-335.

Research output: Contribution to journalReview article

Zainudin, MHM, Hassan, MA, Md Shah, UK, Abdullah, N, Tokura, M, Yasueda, H, Shirai, Y, Sakai, K & Baharuddin, AS 2014, 'Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch', BioResources, vol. 9, no. 1, pp. 316-335.
Zainudin MHM, Hassan MA, Md Shah UK, Abdullah N, Tokura M, Yasueda H et al. Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch. BioResources. 2014 Jan 23;9(1):316-335.
Zainudin, Mohd Huzairi Mohd ; Hassan, Mohd Ali ; Md Shah, Umi Kalsom ; Abdullah, Norhani ; Tokura, Mitsunori ; Yasueda, Hisashi ; Shirai, Yoshihito ; Sakai, Kenji ; Baharuddin, Azhari Samsu. / Bacterial community structure and biochemical changes associated with composting of lignocellulosic oil palm empty fruit bunch. In: BioResources. 2014 ; Vol. 9, No. 1. pp. 316-335.
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