Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics

Zhenhao Ding, Hiromichi Kasahara, Michihiko Nakano, Junya Suehiro

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, an electrical DNA detection method was applied to bacterial detection. DNA was extracted from bacteria and amplified by polymerase chain reaction. The microbeads were labelled with amplicons, altering their surface conductance and therefore their dielectrophoresis characteristics. Amplicon-labelled microbeads could thus be trapped within a high-strength electric field, where they formed a pearl chain between the electrodes, resulting in an increased conductance between the electrodes. This method reduces the amplicon detection time from 1-2 h to 15 min, compared with the conventional method. The presented method realised quantitative detection of specific bacteria at concentrations above 1×105 and 2.4 × 104 CFU/ml for bacterial solutions with and without other bacterial presence, respectively.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalIET Nanobiotechnology
Volume11
Issue number5
DOIs
Publication statusPublished - Aug 1 2017

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Polymerase chain reaction
Electrophoresis
Microspheres
Bacteria
DNA
Polymerase Chain Reaction
Electrodes
Electric fields

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biotechnology
  • Electrical and Electronic Engineering

Cite this

Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics. / Ding, Zhenhao; Kasahara, Hiromichi; Nakano, Michihiko; Suehiro, Junya.

In: IET Nanobiotechnology, Vol. 11, No. 5, 01.08.2017, p. 562-567.

Research output: Contribution to journalArticle

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