Bacterial NanoCellulose Characterization

Tetsuo Kondo, Przemysław Rytczak, Stanislaw Bielecki

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

This section summarizes analytical techniques that are used to characterize Bacterial NanoCellulose (BNC) and presents common physical, chemical methods enabling for detailed description of properties of the native and modified BNC.To characterize structure (determination of length and width of cellulose fibers and crosslinking of the fibers), most often used methods are electron microscopy (EM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM), and CRM Confocal (RAMAN microscopy). Other important parameters that characterize BNC are crystallinity and degree of polymerization (DP). Techniques of cellulose crystallinity measurement involve XRD techniques, solid state 13C NMR, IR infrared spectroscopy, and Raman spectroscopy. Degree of polymerization can be determined by membrane osmometry, cryoscopy, size-exclusion chromatography, ebullioscopy, and determination of reducing-ends concentration. FTIR spectroscopy, GC/MS method, colorimetric analysis (e.g., the phenol-sulfuric method), SEC chromatography, or HPAEC (high performance anion exchange chromatography) can be used for the quantification of carbohydrate component of BNC. Mechanical properties of native and modified BNC usually involve measurement of Young's modulus, per cent elongation at break, and tensile strength.

Original languageEnglish
Title of host publicationBacterial Nanocellulose
Subtitle of host publicationFrom Biotechnology to Bio-Economy
PublisherElsevier Inc.
Pages59-71
Number of pages13
ISBN (Electronic)9780444634665
ISBN (Print)9780444634580
DOIs
Publication statusPublished - Jul 11 2016

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Chromatography
Cellulose
Infrared spectroscopy
Colorimetric analysis
Polymerization
Size exclusion chromatography
Fibers
Confocal microscopy
Phenol
Crosslinking
Anions
Raman spectroscopy
Elongation
Atomic force microscopy
Microscopic examination
Tensile strength
Elastic moduli
Carbohydrates
Nuclear magnetic resonance
Spectroscopy

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Kondo, T., Rytczak, P., & Bielecki, S. (2016). Bacterial NanoCellulose Characterization. In Bacterial Nanocellulose: From Biotechnology to Bio-Economy (pp. 59-71). Elsevier Inc.. https://doi.org/10.1016/B978-0-444-63458-0.00004-4

Bacterial NanoCellulose Characterization. / Kondo, Tetsuo; Rytczak, Przemysław; Bielecki, Stanislaw.

Bacterial Nanocellulose: From Biotechnology to Bio-Economy. Elsevier Inc., 2016. p. 59-71.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kondo, T, Rytczak, P & Bielecki, S 2016, Bacterial NanoCellulose Characterization. in Bacterial Nanocellulose: From Biotechnology to Bio-Economy. Elsevier Inc., pp. 59-71. https://doi.org/10.1016/B978-0-444-63458-0.00004-4
Kondo T, Rytczak P, Bielecki S. Bacterial NanoCellulose Characterization. In Bacterial Nanocellulose: From Biotechnology to Bio-Economy. Elsevier Inc. 2016. p. 59-71 https://doi.org/10.1016/B978-0-444-63458-0.00004-4
Kondo, Tetsuo ; Rytczak, Przemysław ; Bielecki, Stanislaw. / Bacterial NanoCellulose Characterization. Bacterial Nanocellulose: From Biotechnology to Bio-Economy. Elsevier Inc., 2016. pp. 59-71
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