Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions

Young Min Kim, Eiji Yamamoto, Min Sun Kang, Hiroyuki Nakai, Wataru Saburi, Masayuki Okuyama, Haruhide Mori, Kazumi Funane, Mitsuru Momma, Zui Fujimoto, Mikihiko Kobayashi, Doman Kim, Atsuo Kimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.

Original languageEnglish
Pages (from-to)3185-3191
Number of pages7
JournalFEBS Journal
Volume279
Issue number17
DOIs
Publication statusPublished - Sep 1 2012
Externally publishedYes

Fingerprint

cycloisomaltooligosaccharide glucanotransferase
Glycoside Hydrolases
Cyclization
Dextranase
Gene encoding
Enzymes
Ports and harbors
Dextrans
Polymerization
Escherichia coli
Genes
Bacteroides thetaiotaomicron

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions. / Kim, Young Min; Yamamoto, Eiji; Kang, Min Sun; Nakai, Hiroyuki; Saburi, Wataru; Okuyama, Masayuki; Mori, Haruhide; Funane, Kazumi; Momma, Mitsuru; Fujimoto, Zui; Kobayashi, Mikihiko; Kim, Doman; Kimura, Atsuo.

In: FEBS Journal, Vol. 279, No. 17, 01.09.2012, p. 3185-3191.

Research output: Contribution to journalArticle

Kim, YM, Yamamoto, E, Kang, MS, Nakai, H, Saburi, W, Okuyama, M, Mori, H, Funane, K, Momma, M, Fujimoto, Z, Kobayashi, M, Kim, D & Kimura, A 2012, 'Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions', FEBS Journal, vol. 279, no. 17, pp. 3185-3191. https://doi.org/10.1111/j.1742-4658.2012.08698.x
Kim, Young Min ; Yamamoto, Eiji ; Kang, Min Sun ; Nakai, Hiroyuki ; Saburi, Wataru ; Okuyama, Masayuki ; Mori, Haruhide ; Funane, Kazumi ; Momma, Mitsuru ; Fujimoto, Zui ; Kobayashi, Mikihiko ; Kim, Doman ; Kimura, Atsuo. / Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions. In: FEBS Journal. 2012 ; Vol. 279, No. 17. pp. 3185-3191.
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