Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions

Young Min Kim; Eiji Yamamoto; Min Sun Kang; Hiroyuki Nakai; Wataru Saburi; Masayuki Okuyama; Haruhide Mori; Kazumi Funane; Mitsuru Momma; Zui Fujimoto; Mikihiko Kobayashi; Doman Kim; Atsuo Kimura

doi:10.1111/j.1742-4658.2012.08698.x

Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions

Young Min Kim, Eiji Yamamoto, Min Sun Kang, Hiroyuki Nakai, Wataru Saburi, Masayuki Okuyama, Haruhide Mori, Kazumi Funane, Mitsuru Momma, Zui Fujimoto, Mikihiko Kobayashi, Doman Kim, Atsuo Kimura

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.

Original language	English
Pages (from-to)	3185-3191
Number of pages	7
Journal	FEBS Journal
Volume	279
Issue number	17
DOIs	https://doi.org/10.1111/j.1742-4658.2012.08698.x
Publication status	Published - Sept 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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Kim, Y. M., Yamamoto, E., Kang, M. S., Nakai, H., Saburi, W., Okuyama, M., Mori, H., Funane, K., Momma, M., Fujimoto, Z., Kobayashi, M., Kim, D., & Kimura, A. (2012). Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions. FEBS Journal, 279(17), 3185-3191. https://doi.org/10.1111/j.1742-4658.2012.08698.x

Kim, YM, Yamamoto, E, Kang, MS, Nakai, H, Saburi, W, Okuyama, M, Mori, H, Funane, K, Momma, M, Fujimoto, Z, Kobayashi, M, Kim, D & Kimura, A 2012, 'Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions', FEBS Journal, vol. 279, no. 17, pp. 3185-3191. https://doi.org/10.1111/j.1742-4658.2012.08698.x

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title = "Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions",

abstract = "Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.",

author = "Kim, {Young Min} and Eiji Yamamoto and Kang, {Min Sun} and Hiroyuki Nakai and Wataru Saburi and Masayuki Okuyama and Haruhide Mori and Kazumi Funane and Mitsuru Momma and Zui Fujimoto and Mikihiko Kobayashi and Doman Kim and Atsuo Kimura",

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AU - Kim, Young Min

AU - Yamamoto, Eiji

AU - Kang, Min Sun

AU - Nakai, Hiroyuki

AU - Saburi, Wataru

AU - Okuyama, Masayuki

AU - Mori, Haruhide

AU - Funane, Kazumi

AU - Momma, Mitsuru

AU - Fujimoto, Zui

AU - Kobayashi, Mikihiko

AU - Kim, Doman

AU - Kimura, Atsuo

PY - 2012/9

Y1 - 2012/9

N2 - Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.

AB - Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.

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Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions

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