Basal membrane localization of MRP1 in human placental trophoblast

M. Nagashige, F. Ushigome, N. Koyabu, K. Hirata, M. Kawabuchi, T. Hirakawa, S. Satoh, K. Tsukimori, H. Nakano, Takeshi Uchiumi, M. Kuwano, H. Ohtani, Y. Sawada

Research output: Contribution to journalArticle

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Abstract

The placental trophoblast is considered to act as a barrier between mother and fetus, mediating the exchange of various materials across the placenta. ATP-binding cassette (ABC) transporters such as P-glycoprotein (P-gp) and multidrug-resistance protein (MRP) are expressed in the placenta and function as efflux transport systems for xenobiotics. In the present study, we aimed to determine the localization of MRP1 in the human placenta in comparison with that of P-gp. Western blotting analysis with human placental membrane vesicles indicated that P-gp and MRP1 are localized on the brush-border membranes and basal membranes, respectively. Immunohistochemical analysis with human normal full-term placenta showed that anti-P-gp monoclonal antibody F4 stained the brush-border side of the trophoblast cells, whereas anti-MRP1 monoclonal antibody MRPr1 stained the basal side. These results confirm that P-gp and MRP1 are located on the brush-border membranes and basal membranes, respectively, of human full-term placental trophoblast. MRP1 was also detected on the abluminal side of blood vessels in the villi. Accordingly, MRP1 may play a role distinct from that of P-gp, which is considered to restrict the influx of xenobiotics into the fetus.

Original languageEnglish
Pages (from-to)951-958
Number of pages8
JournalPlacenta
Volume24
Issue number10
DOIs
Publication statusPublished - Jan 1 2003

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Trophoblasts
P-Glycoprotein
Placenta
Membranes
Microvilli
Xenobiotics
Fetus
Monoclonal Antibodies
P-Glycoproteins
ATP-Binding Cassette Transporters
Blood Vessels
Western Blotting

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

Cite this

Nagashige, M., Ushigome, F., Koyabu, N., Hirata, K., Kawabuchi, M., Hirakawa, T., ... Sawada, Y. (2003). Basal membrane localization of MRP1 in human placental trophoblast. Placenta, 24(10), 951-958. https://doi.org/10.1016/S0143-4004(03)00170-X

Basal membrane localization of MRP1 in human placental trophoblast. / Nagashige, M.; Ushigome, F.; Koyabu, N.; Hirata, K.; Kawabuchi, M.; Hirakawa, T.; Satoh, S.; Tsukimori, K.; Nakano, H.; Uchiumi, Takeshi; Kuwano, M.; Ohtani, H.; Sawada, Y.

In: Placenta, Vol. 24, No. 10, 01.01.2003, p. 951-958.

Research output: Contribution to journalArticle

Nagashige, M, Ushigome, F, Koyabu, N, Hirata, K, Kawabuchi, M, Hirakawa, T, Satoh, S, Tsukimori, K, Nakano, H, Uchiumi, T, Kuwano, M, Ohtani, H & Sawada, Y 2003, 'Basal membrane localization of MRP1 in human placental trophoblast', Placenta, vol. 24, no. 10, pp. 951-958. https://doi.org/10.1016/S0143-4004(03)00170-X
Nagashige M, Ushigome F, Koyabu N, Hirata K, Kawabuchi M, Hirakawa T et al. Basal membrane localization of MRP1 in human placental trophoblast. Placenta. 2003 Jan 1;24(10):951-958. https://doi.org/10.1016/S0143-4004(03)00170-X
Nagashige, M. ; Ushigome, F. ; Koyabu, N. ; Hirata, K. ; Kawabuchi, M. ; Hirakawa, T. ; Satoh, S. ; Tsukimori, K. ; Nakano, H. ; Uchiumi, Takeshi ; Kuwano, M. ; Ohtani, H. ; Sawada, Y. / Basal membrane localization of MRP1 in human placental trophoblast. In: Placenta. 2003 ; Vol. 24, No. 10. pp. 951-958.
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