Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering

Nana Shirakigawa, Takayuki Takei, Hiroyuki Ijima

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.

Original languageEnglish
Pages (from-to)740-745
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume116
Issue number6
DOIs
Publication statusPublished - Dec 1 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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