Ba2+ current oscillations modulated by cyclic AMP and phorbol esters in ras-transformed fibroblasts

Haruhiro Higashida, Naoto Hoshi, Mami Noda, Mohammad Shahidullah, Minako Hashii, Yoshinori Nozawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An oscillatory influx of divalent cations was measured as Ba2+ inward currents (Ba2+ current oscillations) by voltage-clamp recording in v-Ki-ras-transformed NIH 3T3 (DT) fibroblasts after activation with bradykinin or serum. Application of forskolin or dibutyryl cyclic AMP onto DT cells initiated Ba2+ current oscillations. Increasing intracellular cyclic AMP reduced the amplitude but increased the frequency of the Ba2+ current oscillations. Activation of protein kinase C by phorbol esters terminated Ba2+ current oscillations. No inhibition of Ba2+ current oscillations by phorbol esters was observed in down-regulated cells that had been pretreated with phorbol esters for 24 hrs. The results suggest that Ba2+ current oscillations are regulated by intracellular second messengers.

Original languageEnglish
Pages (from-to)1240-1245
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number3
DOIs
Publication statusPublished - Feb 14 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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