Bax-inhibiting peptide attenuates bleomycin-induced lung injury in mice

Kunihiro Suzuki, Toyoshi Yanagihara, Tetsuya Yokoyama, Takashige Maeyama, Saiko Ogata-Suetsugu, Masako Arimura-Omori, Hironori Mikumo, Naoki Hamada, Eiji Harada, Kazuyoshi Kuwano, Taishi Harada, Yoichi Nakanishi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bax is a pro-apoptotic member of the Bcl-2 family of proteins, and plays a central role in mitochondria-dependent apoptosis. Several lines of evidence have implied that Bax is involved in both epithelial apoptosis and fibroblast proliferation in idiopathic pulmonary fibrosis; however, the mechanisms remain unknown. Bax-inhibiting peptide V5 (BIP-V5) exhibits membrane permeability and inhibits the activation of Bax. The purpose of this study was to investigate whether the control of Bax activity by BIP-V5 reduces the degree of bleomycin-induced lung injury. C57BL/6J mice were administered bleomycin and BIP-V5 intratracheally on day 0. Bronchoalveolar lavage fluid and lung tissue were obtained on day 7. Human pulmonary alveolar epithelial cells (A549 cells) and mouse pulmonary alveolar epithelial cells (LA-4 cells) were stimulated with bleomycin to induce apoptosis. Administration of BIP-V5 improved the survival rate and degree of bleomycin-induced lung injury by suppressing Bax activation in mice. BIP-V5 treatment decreased bleomycin-induced apoptosis of alveolar epithelial cell lines (A549 cells and LA-4 cells) by suppressing Bax activation. These results indicate that administration of BIP-V5 may constitute a novel therapeutic strategy against lung injury.

Original languageEnglish
Pages (from-to)1869-1875
Number of pages7
JournalBiology Open
Volume6
Issue number12
DOIs
Publication statusPublished - Dec 15 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Bax-inhibiting peptide attenuates bleomycin-induced lung injury in mice'. Together they form a unique fingerprint.

  • Cite this

    Suzuki, K., Yanagihara, T., Yokoyama, T., Maeyama, T., Ogata-Suetsugu, S., Arimura-Omori, M., Mikumo, H., Hamada, N., Harada, E., Kuwano, K., Harada, T., & Nakanishi, Y. (2017). Bax-inhibiting peptide attenuates bleomycin-induced lung injury in mice. Biology Open, 6(12), 1869-1875. https://doi.org/10.1242/bio.026005