Behavior of a calcium phosphate cement in simulated blood plasma in vitro

Kunio Ishikawa, Shozo Takagi, Laurence C. Chow, Yoshiko Ishikawa, Edward D. Eanes, Kenzo Asaoka

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Objectives. The purpose of this study was to gain a better understanding of the integration of calcium phosphate cement (CPC) implants in biological tissue. Methods. An in vitro continuous flow system was employed to examine the protracted behavior of disc-shaped specimens of this bioactive material under sustained physiological-like solution conditions. Weight measurement, diametral tensile strength measurement (DTS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and powder x-ray diffraction (XRD) were used to characterize the CPC samples as a function of immersion time. Results. When CPC was immersed in simulated blood plasma in which the Ca (2.5 mmol/L) and inorganic phosphate (1.0 mmol/L levels were kept constant, both the weight and DTS of the specimens steadily increased to about 1.5 times their original values over a period of 20 wk. SEM obsevations showed new precipitate formations in intimate contact with the original CPC surface. FTIR and XRD analyses revealed that the precipitate was a B-type carbonate hydroxyapatite (OHAp), the type of OHAp observed in bone and dentin. On the other hand, the interior of CPC discs did not show an increase in either bulk density or OHAp content. Thus, the increases in weight and DTS are attributal to the OHAp precipitation on the CPC surface. Significance. The results suggest that under in vivo conditions, CPC implants would not dissolve in physiological fluids. OHAp coatings may form on the implants, which may enhance bonding of implants to bone by mechanically strengthening the interface between them.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalDental Materials
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1994
Externally publishedYes

Fingerprint

Calcium phosphate
Cements
Blood
Plasmas
Tensile Strength
Tensile strength
Fourier Transform Infrared Spectroscopy
Weights and Measures
Electron Scanning Microscopy
Fourier transform infrared spectroscopy
Precipitates
Bone
Diffraction
X-Rays
Bone and Bones
X rays
Interiors (building)
Scanning electron microscopy
Carbonates
Immersion

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Behavior of a calcium phosphate cement in simulated blood plasma in vitro. / Ishikawa, Kunio; Takagi, Shozo; Chow, Laurence C.; Ishikawa, Yoshiko; Eanes, Edward D.; Asaoka, Kenzo.

In: Dental Materials, Vol. 10, No. 1, 01.1994, p. 26-32.

Research output: Contribution to journalArticle

Ishikawa, K, Takagi, S, Chow, LC, Ishikawa, Y, Eanes, ED & Asaoka, K 1994, 'Behavior of a calcium phosphate cement in simulated blood plasma in vitro', Dental Materials, vol. 10, no. 1, pp. 26-32. https://doi.org/10.1016/0109-5641(94)90018-3
Ishikawa, Kunio ; Takagi, Shozo ; Chow, Laurence C. ; Ishikawa, Yoshiko ; Eanes, Edward D. ; Asaoka, Kenzo. / Behavior of a calcium phosphate cement in simulated blood plasma in vitro. In: Dental Materials. 1994 ; Vol. 10, No. 1. pp. 26-32.
@article{5a577c71fa154fd29bb294205a860ac8,
title = "Behavior of a calcium phosphate cement in simulated blood plasma in vitro",
abstract = "Objectives. The purpose of this study was to gain a better understanding of the integration of calcium phosphate cement (CPC) implants in biological tissue. Methods. An in vitro continuous flow system was employed to examine the protracted behavior of disc-shaped specimens of this bioactive material under sustained physiological-like solution conditions. Weight measurement, diametral tensile strength measurement (DTS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and powder x-ray diffraction (XRD) were used to characterize the CPC samples as a function of immersion time. Results. When CPC was immersed in simulated blood plasma in which the Ca (2.5 mmol/L) and inorganic phosphate (1.0 mmol/L levels were kept constant, both the weight and DTS of the specimens steadily increased to about 1.5 times their original values over a period of 20 wk. SEM obsevations showed new precipitate formations in intimate contact with the original CPC surface. FTIR and XRD analyses revealed that the precipitate was a B-type carbonate hydroxyapatite (OHAp), the type of OHAp observed in bone and dentin. On the other hand, the interior of CPC discs did not show an increase in either bulk density or OHAp content. Thus, the increases in weight and DTS are attributal to the OHAp precipitation on the CPC surface. Significance. The results suggest that under in vivo conditions, CPC implants would not dissolve in physiological fluids. OHAp coatings may form on the implants, which may enhance bonding of implants to bone by mechanically strengthening the interface between them.",
author = "Kunio Ishikawa and Shozo Takagi and Chow, {Laurence C.} and Yoshiko Ishikawa and Eanes, {Edward D.} and Kenzo Asaoka",
year = "1994",
month = "1",
doi = "10.1016/0109-5641(94)90018-3",
language = "English",
volume = "10",
pages = "26--32",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "1",

}

TY - JOUR

T1 - Behavior of a calcium phosphate cement in simulated blood plasma in vitro

AU - Ishikawa, Kunio

AU - Takagi, Shozo

AU - Chow, Laurence C.

AU - Ishikawa, Yoshiko

AU - Eanes, Edward D.

AU - Asaoka, Kenzo

PY - 1994/1

Y1 - 1994/1

N2 - Objectives. The purpose of this study was to gain a better understanding of the integration of calcium phosphate cement (CPC) implants in biological tissue. Methods. An in vitro continuous flow system was employed to examine the protracted behavior of disc-shaped specimens of this bioactive material under sustained physiological-like solution conditions. Weight measurement, diametral tensile strength measurement (DTS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and powder x-ray diffraction (XRD) were used to characterize the CPC samples as a function of immersion time. Results. When CPC was immersed in simulated blood plasma in which the Ca (2.5 mmol/L) and inorganic phosphate (1.0 mmol/L levels were kept constant, both the weight and DTS of the specimens steadily increased to about 1.5 times their original values over a period of 20 wk. SEM obsevations showed new precipitate formations in intimate contact with the original CPC surface. FTIR and XRD analyses revealed that the precipitate was a B-type carbonate hydroxyapatite (OHAp), the type of OHAp observed in bone and dentin. On the other hand, the interior of CPC discs did not show an increase in either bulk density or OHAp content. Thus, the increases in weight and DTS are attributal to the OHAp precipitation on the CPC surface. Significance. The results suggest that under in vivo conditions, CPC implants would not dissolve in physiological fluids. OHAp coatings may form on the implants, which may enhance bonding of implants to bone by mechanically strengthening the interface between them.

AB - Objectives. The purpose of this study was to gain a better understanding of the integration of calcium phosphate cement (CPC) implants in biological tissue. Methods. An in vitro continuous flow system was employed to examine the protracted behavior of disc-shaped specimens of this bioactive material under sustained physiological-like solution conditions. Weight measurement, diametral tensile strength measurement (DTS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and powder x-ray diffraction (XRD) were used to characterize the CPC samples as a function of immersion time. Results. When CPC was immersed in simulated blood plasma in which the Ca (2.5 mmol/L) and inorganic phosphate (1.0 mmol/L levels were kept constant, both the weight and DTS of the specimens steadily increased to about 1.5 times their original values over a period of 20 wk. SEM obsevations showed new precipitate formations in intimate contact with the original CPC surface. FTIR and XRD analyses revealed that the precipitate was a B-type carbonate hydroxyapatite (OHAp), the type of OHAp observed in bone and dentin. On the other hand, the interior of CPC discs did not show an increase in either bulk density or OHAp content. Thus, the increases in weight and DTS are attributal to the OHAp precipitation on the CPC surface. Significance. The results suggest that under in vivo conditions, CPC implants would not dissolve in physiological fluids. OHAp coatings may form on the implants, which may enhance bonding of implants to bone by mechanically strengthening the interface between them.

UR - http://www.scopus.com/inward/record.url?scp=0028367265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028367265&partnerID=8YFLogxK

U2 - 10.1016/0109-5641(94)90018-3

DO - 10.1016/0109-5641(94)90018-3

M3 - Article

C2 - 7995472

AN - SCOPUS:0028367265

VL - 10

SP - 26

EP - 32

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 1

ER -