Behavioral abnormalities and reduced norepinephrine in EP4 receptor-associated protein (EPRAP)-deficient mice

Risako Fujikawa, Sei Higuchi, Taichi Ikedo, Manabu Nagata, Kosuke Hayashi, Tao Yang, Takeshi Miyata, Masayuki Yokode, Manabu Minami

Research output: Contribution to journalArticle

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Abstract

EP4 receptor-associated protein (EPRAP) is a newly identified molecule that regulates macrophage activation. We recently demonstrated the presence of EPRAP in the mice brain; however, little is known about the function of EPRAP in this tissue. Therefore, we investigated the role of EPRAP in behavior and emotion using behavioral analysis in mice. In this study, we subjected EPRAP-deficient (KO) mice and wild-type C57BL/6 (WT) mice to a battery of behavioral tests. EPRAP-KO mice tended to have shorter latencies to fall in the wire hang test, but had normal neuromuscular strength. EPRAP-KO mice exhibited elevated startle responses and reduced pre-pulse inhibition. Compared with WT mice, EPRAP-KO mice increased depression-like behavior in the forced swim test. These abnormal behaviors partially mimic symptoms of depression, attention deficit hyperactivity disorder (ADHD) and schizophrenia. Methylphenidate administration increased locomotor activity less in EPRAP-KO mice than in WT mice. Finally, levels of norepinephrine were reduced in the EPRAP-KO mouse brain. These behavioral abnormalities in EPRAP-KO mice may result from the dysfunction of monoamines, in particular, norepinephrine. Our results suggest that EPRAP participates in the pathogenesis of various behavioral disorders.

Original languageEnglish
Pages (from-to)584-588
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume486
Issue number2
DOIs
Publication statusPublished - Apr 29 2017

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Norepinephrine
Proteins
Inbred C57BL Mouse
Brain
Startle Reflex
Depression
Methylphenidate
Macrophage Activation
Macrophages
Locomotion
Attention Deficit Disorder with Hyperactivity
Schizophrenia
Emotions
Chemical activation
Wire
Tissue
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Behavioral abnormalities and reduced norepinephrine in EP4 receptor-associated protein (EPRAP)-deficient mice. / Fujikawa, Risako; Higuchi, Sei; Ikedo, Taichi; Nagata, Manabu; Hayashi, Kosuke; Yang, Tao; Miyata, Takeshi; Yokode, Masayuki; Minami, Manabu.

In: Biochemical and Biophysical Research Communications, Vol. 486, No. 2, 29.04.2017, p. 584-588.

Research output: Contribution to journalArticle

Fujikawa, Risako ; Higuchi, Sei ; Ikedo, Taichi ; Nagata, Manabu ; Hayashi, Kosuke ; Yang, Tao ; Miyata, Takeshi ; Yokode, Masayuki ; Minami, Manabu. / Behavioral abnormalities and reduced norepinephrine in EP4 receptor-associated protein (EPRAP)-deficient mice. In: Biochemical and Biophysical Research Communications. 2017 ; Vol. 486, No. 2. pp. 584-588.
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