Behavioral defects in a DCTN1G71A transgenic mouse model of Perry syndrome

Takayasu Mishima, Manami Deshimaru, Takuya Watanabe, Kaori Kubota, Mariko Kinoshita-Kawada, Junichi Yuasa-Kawada, Kotaro Takasaki, Yoshinari Uehara, Shozo Jinno, Katsunori Iwasaki, Yoshio Tsuboi

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2 Citations (Scopus)

Abstract

Perry syndrome is a rare neurodegenerative disease characterized by parkinsonism, depression/apathy, weight loss, and central hypoventilation. Our previously-conducted genome-wide association scan and subsequent studies identified nine mutations in DCTN1, the largest protein subunit of the dynactin complex, in patients with Perry syndrome. These included G71A in the microtubule-binding cytoskeleton-associated protein Gly-rich domain of p150Glued. The dynactin complex is essential for function of the microtubule-based cytoplasmic retrograde motor dynein. To test the hypothesis that the G71A mutation in the DCTN1 gene is sufficient to cause Perry syndrome, we generated DCTN1G71A transgenic mice. These mice initially developed normally, but young animals showed decreased exploratory activity and aged animals showed impaired motor coordination. These behavioral defects parallel apathy-like symptoms and parkinsonism encountered in Perry syndrome. TDP-43 aggregates were not detected in the substantia nigra and cerebral cortex of the transgenic mice, although pathological aggregates of TDP-43 have been considered a major neuropathological feature of Perry syndrome. Our study reveals that a single mutation in the DCTN1 gene recapitulates symptoms of Perry syndrome patients, and provides evidence that DCTN1G71A transgenic mice represent a novel rodent model of Perry syndrome.

Original languageEnglish
Pages (from-to)98-103
Number of pages6
JournalNeuroscience Letters
Volume666
DOIs
Publication statusPublished - Feb 14 2018

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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    Mishima, T., Deshimaru, M., Watanabe, T., Kubota, K., Kinoshita-Kawada, M., Yuasa-Kawada, J., Takasaki, K., Uehara, Y., Jinno, S., Iwasaki, K., & Tsuboi, Y. (2018). Behavioral defects in a DCTN1G71A transgenic mouse model of Perry syndrome. Neuroscience Letters, 666, 98-103. https://doi.org/10.1016/j.neulet.2017.12.038