Beneficial effect of MCI-186 on hepatic warm ischemia-reperfusion in the rat

Mizuki Ninomiya, Mitsuo Shimada, Noboru Harada, Satoko Shiotani, Shoji Hiroshige, Yuji Soejima, Taketoshi Suehiro, Keizo Sugimachi

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Abstract

Despite growing evidence that reactive oxygen species are responsible for deleterious effects of ischemia-reperfusion (I/R) injury of the liver, there exists, until now, no reliable antioxidant therapeutics applicable in the clinical setting. We investigated the effects of free radical scavenger, 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), using an isolated liver perfusion model to elucidate its possible therapeutic effects on hepatic warm I/R injury. Isolated livers from Wistar rats were reperfused for 120 min with an oxygenated KrebsHenseleit bicarbonate buffer after 1 hr of warm ischemia. Addition of MCI-186 (1 mg/L) into the perfusate significantly improved portal flow, hepatic enzyme release into the perfusate, total bile production, histologic alteration, and malondialdehyde concentration but not sinusoidal endothelial cell function as assessed by the clearance of hyaluronic acid. MCI-186 seems to have protective effects against hepatic warm I/R injury by attenuating the damage of the hepatocyte, which is the major target of oxidative damage in this model.

Original languageEnglish
Pages (from-to)1470-1472
Number of pages3
JournalTransplantation
Volume74
Issue number10
DOIs
Publication statusPublished - Nov 1 2002

All Science Journal Classification (ASJC) codes

  • Transplantation

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    Ninomiya, M., Shimada, M., Harada, N., Shiotani, S., Hiroshige, S., Soejima, Y., Suehiro, T., & Sugimachi, K. (2002). Beneficial effect of MCI-186 on hepatic warm ischemia-reperfusion in the rat. Transplantation, 74(10), 1470-1472. https://doi.org/10.1097/00007890-200211270-00021