Beneficial effects of splenectomy on massive hepatectomy model in rats

Yusuke Arakawa, Mitsuo Shimada, Hideaki Uchiyama, Toru Ikegami, Tomoharu Yoshizumi, Satoru Imura, Yuji Morine, Hirohumi Kanemura

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21 Citations (Scopus)


Aim: Possible spleno-hepatic relationships during hepatectomy remain unclear. The purpose of this study was to investigate the impact of splenectomy during massive hepatectomy in rats. Methods: Rats were divided into the following two groups: 90% hepatectomy (Hx group), hepatectomy with splenectomy (Hx+Sp group). The following parameters were evaluated; survival rate, biochemical parameters, quantitative RT-PCR for hemeoxygenase-1 (HO-1) and tumor necrosing factor α (TNFα), immunohistochemical staining for HO-1, proliferating cell nuclear antigen labeling index and liver weights. Results: The survival rate after massive hepatectomy significantly improved in Hx+Sp group as well as serum biochemical parameters, compared with Hx group (P < 0.05). HO-1 positive hepatocytes and its mRNA expression significantly increased and TNFα mRNA expression significantly decreased in Hx+Sp group compared with Hx group (P < 0.05). Moreover, liver regeneration was significantly accelerated at 48 and 72 h after hepatectomy in Hx+Sp group. Conclusions: Splenectomy had beneficial effects on massive hepatectomy by ameliorating liver injuries and promoting preferable liver regeneration.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalHepatology Research
Issue number4
Publication statusPublished - Apr 8 2009
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Hepatology
  • Infectious Diseases

Cite this

Arakawa, Y., Shimada, M., Uchiyama, H., Ikegami, T., Yoshizumi, T., Imura, S., ... Kanemura, H. (2009). Beneficial effects of splenectomy on massive hepatectomy model in rats. Hepatology Research, 39(4), 391-397.