Beneficial effects of splenectomy on massive hepatectomy model in rats

Yusuke Arakawa, Mitsuo Shimada, Hideaki Uchiyama, Toru Ikegami, Tomoharu Yoshizumi, Satoru Imura, Yuji Morine, Hirohumi Kanemura

Research output: Contribution to journalArticle

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Abstract

Aim: Possible spleno-hepatic relationships during hepatectomy remain unclear. The purpose of this study was to investigate the impact of splenectomy during massive hepatectomy in rats. Methods: Rats were divided into the following two groups: 90% hepatectomy (Hx group), hepatectomy with splenectomy (Hx+Sp group). The following parameters were evaluated; survival rate, biochemical parameters, quantitative RT-PCR for hemeoxygenase-1 (HO-1) and tumor necrosing factor α (TNFα), immunohistochemical staining for HO-1, proliferating cell nuclear antigen labeling index and liver weights. Results: The survival rate after massive hepatectomy significantly improved in Hx+Sp group as well as serum biochemical parameters, compared with Hx group (P < 0.05). HO-1 positive hepatocytes and its mRNA expression significantly increased and TNFα mRNA expression significantly decreased in Hx+Sp group compared with Hx group (P < 0.05). Moreover, liver regeneration was significantly accelerated at 48 and 72 h after hepatectomy in Hx+Sp group. Conclusions: Splenectomy had beneficial effects on massive hepatectomy by ameliorating liver injuries and promoting preferable liver regeneration.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalHepatology Research
Volume39
Issue number4
DOIs
Publication statusPublished - Apr 8 2009

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Hepatectomy
Splenectomy
Heme Oxygenase-1
Liver Regeneration
Liver
Messenger RNA
Proliferating Cell Nuclear Antigen
Hepatocytes
Neoplasms
Staining and Labeling
Weights and Measures
Polymerase Chain Reaction
Wounds and Injuries
Serum

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Infectious Diseases

Cite this

Beneficial effects of splenectomy on massive hepatectomy model in rats. / Arakawa, Yusuke; Shimada, Mitsuo; Uchiyama, Hideaki; Ikegami, Toru; Yoshizumi, Tomoharu; Imura, Satoru; Morine, Yuji; Kanemura, Hirohumi.

In: Hepatology Research, Vol. 39, No. 4, 08.04.2009, p. 391-397.

Research output: Contribution to journalArticle

Arakawa, Y, Shimada, M, Uchiyama, H, Ikegami, T, Yoshizumi, T, Imura, S, Morine, Y & Kanemura, H 2009, 'Beneficial effects of splenectomy on massive hepatectomy model in rats', Hepatology Research, vol. 39, no. 4, pp. 391-397. https://doi.org/10.1111/j.1872-034X.2008.00469.x
Arakawa, Yusuke ; Shimada, Mitsuo ; Uchiyama, Hideaki ; Ikegami, Toru ; Yoshizumi, Tomoharu ; Imura, Satoru ; Morine, Yuji ; Kanemura, Hirohumi. / Beneficial effects of splenectomy on massive hepatectomy model in rats. In: Hepatology Research. 2009 ; Vol. 39, No. 4. pp. 391-397.
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