Bessel-beam illumination Raman microscopy

Kazuki Bando; Shumpei Yabuuchi; Menglu Li; Toshiki Kubo; Ryosuke Oketani; Nicholas I. Smith; Katsumasa Fujita

doi:10.1364/BOE.456138

Bessel-beam illumination Raman microscopy

Kazuki Bando, Shumpei Yabuuchi, Menglu Li, Toshiki Kubo, Ryosuke Oketani, Nicholas I. Smith, Katsumasa Fujita

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

Original language	English
Pages (from-to)	3161-3170
Number of pages	10
Journal	Biomedical Optics Express
Volume	13
Issue number	6
DOIs	https://doi.org/10.1364/BOE.456138
Publication status	Published - Jun 1 2022
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.456138

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title = "Bessel-beam illumination Raman microscopy",

abstract = "We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination. ",

author = "Kazuki Bando and Shumpei Yabuuchi and Menglu Li and Toshiki Kubo and Ryosuke Oketani and Smith, {Nicholas I.} and Katsumasa Fujita",

note = "Funding Information: Acknowledgments. The authors thank Ryosuke Morisaki, Tomoyuki Murashima, and Takuma Shibata for advice on sensitivity evaluation and optical setup construction. This work was partially supported by JST-Mirai Program and JST-CREST program under Grant Numbers JPMJMI20G3 and JPMJCR1925, Japan. Publisher Copyright: {\textcopyright} 2022 Optica Publishing Group.",

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doi = "10.1364/BOE.456138",

language = "English",

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TY - JOUR

T1 - Bessel-beam illumination Raman microscopy

AU - Bando, Kazuki

AU - Yabuuchi, Shumpei

AU - Li, Menglu

AU - Kubo, Toshiki

AU - Oketani, Ryosuke

AU - Smith, Nicholas I.

AU - Fujita, Katsumasa

N1 - Funding Information: Acknowledgments. The authors thank Ryosuke Morisaki, Tomoyuki Murashima, and Takuma Shibata for advice on sensitivity evaluation and optical setup construction. This work was partially supported by JST-Mirai Program and JST-CREST program under Grant Numbers JPMJMI20G3 and JPMJCR1925, Japan. Publisher Copyright: © 2022 Optica Publishing Group.

PY - 2022/6/1

Y1 - 2022/6/1

N2 - We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

AB - We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

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DO - 10.1364/BOE.456138

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 6

ER -

Bessel-beam illumination Raman microscopy

Abstract

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