bHLH-ORANGE family genes regulate the expression of E-box clock genes in Drosophila

Taichi Itoh, Teiichi Tanimura, Akira Matsumoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Drosophila has 13 basic helix-loop-helix-ORANGE (bHLH-O) family genes. One of the members, clockwork orange (cwo), which is the counterpart of mammalian clock genes Dec1 and Dec2, regulates the transcriptional feedback loops of circadian clock genes through binding to E-box sequences in target gene promoters. The goal of the current study was to determine the role of Drosophila bHLH-O proteins in circadian rhythms at the molecular and behavioral level. Promoter assays in cultured Drosophila S2 cells were carried out to investigate which of the known bHLH-O proteins directly regulates the transcription of clock genes. In addition to CWO, three other bHLH-O proteins, SIDE, Mβ and Mγ, suppressed E-box clock gene transcription in vitro. RNA interference (RNAi) was used to generate bHLH-O knockdown flies in pacemaker neurons, and then locomotor activity rhythm was measured. cwo knockdown flies exhibited a remarkable phenotype. To clarify the functional complementation in circadian regulation among CWO, SIDE, Mβ and Mγ, promoter activity in the presence of combinations of two bHLH-O genes and locomotor rhythm in double knockdown flies were examined. The results suggest that CWO predominantly acts as a key factor of circadian regulation both in vitro and in vivo.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalApplied Entomology and Zoology
Volume46
Issue number3
DOIs
Publication statusPublished - Aug 1 2011

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Drosophila
gene expression
genes
promoter regions
circadian rhythm
transcription (genetics)
proteins
RNA interference
locomotion
neurons
phenotype
assays
cells

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

bHLH-ORANGE family genes regulate the expression of E-box clock genes in Drosophila. / Itoh, Taichi; Tanimura, Teiichi; Matsumoto, Akira.

In: Applied Entomology and Zoology, Vol. 46, No. 3, 01.08.2011, p. 391-397.

Research output: Contribution to journalArticle

Itoh, Taichi ; Tanimura, Teiichi ; Matsumoto, Akira. / bHLH-ORANGE family genes regulate the expression of E-box clock genes in Drosophila. In: Applied Entomology and Zoology. 2011 ; Vol. 46, No. 3. pp. 391-397.
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